C57BL/6JCya-Ilf3em1flox/Cya
Common Name:
Ilf3-flox
Product ID:
S-CKO-19314
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ilf3-flox
Strain ID
CKOCMP-16201-Ilf3-B6J-VB
Gene Name
Product ID
S-CKO-19314
Gene Alias
MBII-26; MPHOSPH4; NF90; NFAR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ilf3em1flox/Cya mice (Catalog S-CKO-19314) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115414
NCBI RefSeq
NM_001042709
Target Region
Exon 5~11
Size of Effective Region
~5.1 kb
Detailed Document
Overview of Gene Research
Ilf3, also known as Interleukin enhancer-binding factor 3, is a gene with diverse functions. It encodes double-stranded RNA-binding proteins that are involved in multiple RNA metabolism processes. Ilf3 is implicated in regulating the cell cycle, enzymatic activities, and is associated with pathways like mTORC1-dependent amino acid sensing, innate immune response, and serine biosynthesis [1,3,4].
Macrophage-conditional Ilf3 knockout (Ilf3M-KO) and transgenic (Ilf3M-Tg) mice, as well as smooth muscle-conditional Ilf3 knockout (Ilf3SM-KO) and transgenic (Ilf3SM-Tg) mice, have been used to study its functions. In macrophages, Ilf3 deficiency attenuates abdominal aortic aneurysm progression, while its elevation exacerbates the lesions. Mechanistically, it increases NF-κB activity and represses the anti-inflammatory action [2]. In atherosclerotic calcification, hyperlipidemia increases Ilf3 expression, and its deletion in relevant cell types may prevent and reverse the process by regulating BMP2 and STAT1 transcription [5].
In conclusion, Ilf3 plays crucial roles in multiple biological processes and disease conditions. The use of Ilf3 KO/CKO mouse models has revealed its significance in abdominal aortic aneurysm development and atherosclerotic calcification, providing potential therapeutic targets for these diseases.
References:
1. Yan, Guokai, Yang, Jinxin, Li, Wen, Guan, Jialiang, Liu, Ying. 2023. Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing. In Nature cell biology, 25, 754-764. doi:10.1038/s41556-023-01123-x. https://pubmed.ncbi.nlm.nih.gov/37037994/
2. Wang, Zhao-Yang, Cheng, Jie, Wang, Ying, Chen, Yu-Guo, Zhang, Ming-Xiang. 2024. Macrophage ILF3 promotes abdominal aortic aneurysm by inducing inflammatory imbalance in male mice. In Nature communications, 15, 7249. doi:10.1038/s41467-024-51030-4. https://pubmed.ncbi.nlm.nih.gov/39179537/
3. Castella, Sandrine, Bernard, Rozenn, Corno, Mélanie, Fradin, Aurélie, Larcher, Jean-Christophe. 2014. Ilf3 and NF90 functions in RNA biology. In Wiley interdisciplinary reviews. RNA, 6, 243-56. doi:10.1002/wrna.1270. https://pubmed.ncbi.nlm.nih.gov/25327818/
4. Li, Kai, Wu, Jian-Lin, Qin, Baifu, Fang, Lekun, Lee, Mong-Hong. 2019. ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer. In Cell research, 30, 163-178. doi:10.1038/s41422-019-0257-1. https://pubmed.ncbi.nlm.nih.gov/31772275/
5. Xie, Fei, Cui, Qing-Ke, Wang, Zhao-Yang, Wang, Ying, Zhang, Ming-Xiang. 2021. ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription. In Journal of molecular and cellular cardiology, 161, 39-52. doi:10.1016/j.yjmcc.2021.07.011. https://pubmed.ncbi.nlm.nih.gov/34343541/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen