C57BL/6JCya-Kntc1em1flox/Cya
Common Name
Kntc1-flox
Product ID
S-CKO-19320
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-208628-Kntc1-B6J-VB
When using this mouse strain in a publication, please cite “Kntc1-flox Mouse (Catalog S-CKO-19320) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Kntc1-flox
Strain ID
CKOCMP-208628-Kntc1-B6J-VB
Gene Name
Product ID
S-CKO-19320
Gene Alias
D330012D13, jgl
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031366
NCBI RefSeq
NM_001042421
Target Region
Exon 3~6
Size of Effective Region
~4.5 kb
Overview of Gene Research
KNTC1, kinetochore associated 1, is a protein involved in chromosome segregation, being a component of the Rod-Zwilch-ZW10 (RZZ) complex essential for sister chromatid segregation during mitosis and participating in the spindle checkpoint [5]. It may also be associated with pathways like PI3K/Akt/mTOR [1]. Understanding its function is crucial for clarifying cell division-related biological processes.
In multiple cancers, loss-of-function studies have shown significant impacts. In gastric cancer, KNTC1 knockdown suppressed cell proliferation and migration while promoting apoptosis, potentially via the PI3K/Akt/mTOR pathway [1]. In liver cancer, it inhibited cell viability, migration, and invasion, arrested the cell cycle at the S-phase through CDK1 modulation, and decreased tumor growth in mouse transplanted tumors [2]. In pancreatic cancer, KNTC1 knockdown decreased cell growth and increased apoptosis [3]. In colon cancer, it reduced cell growth rates, increased apoptosis, and arrested cells in the S phase [4]. In non-small-cell lung cancer, it restrained cell viability, proliferation, migration, and invasion, and inhibited tumorigenesis in a xenograft model [6].
In conclusion, KNTC1 plays a significant role in cell division-related biological processes. Through gene knockout or knockdown models in various cancer types such as gastric, liver, pancreatic, colon, and non-small-cell lung cancers, it has been revealed that KNTC1 promotes cancer cell proliferation, migration, and tumor growth, making it a potential biomarker and therapeutic target for these cancers.
References：
1. Qi, Haoran, Cao, Mengyuan, Chen, Yanan, Duan, Xiaoxuan, Lu, Jing. 2024. KNTC1 functions as a potential biomarker and oncogene regulating proliferation, migration and apoptosis in gastric cancer. In International immunopharmacology, 143, 113257. doi:10.1016/j.intimp.2024.113257. https://pubmed.ncbi.nlm.nih.gov/39362011/
2. Yang, Fan, Yuan, Changjin. 2023. KNTC1 knockdown inhibits proliferation and metastases of liver cancer. In 3 Biotech, 13, 309. doi:10.1007/s13205-023-03722-9. https://pubmed.ncbi.nlm.nih.gov/37621322/
3. Liu, Ling, Chen, Hongwei, Chen, Xinan, Shen, Weimin, Jia, Changku. 2022. KNTC1 as a putative tumor oncogene in pancreatic cancer. In Journal of cancer research and clinical oncology, 149, 3023-3031. doi:10.1007/s00432-022-04146-3. https://pubmed.ncbi.nlm.nih.gov/35852618/
4. Zhengxiang, Zhang, Yunxiang, Tao, Zhiping, Liu, Zhimin, Yang. 2021. KNTC1 knockdown suppresses cell proliferation of colon cancer. In 3 Biotech, 11, 262. doi:10.1007/s13205-021-02800-0. https://pubmed.ncbi.nlm.nih.gov/33996374/
5. Tong, Hui, Liu, Xiaohui, Peng, Chenghong, Shen, Baiyong, Zhu, Zhecheng. 2022. Silencing of KNTC1 inhibits hepatocellular carcinoma cells progression via suppressing PI3K/Akt pathway. In Cellular signalling, 101, 110498. doi:10.1016/j.cellsig.2022.110498. https://pubmed.ncbi.nlm.nih.gov/36273753/
6. Liu, Ruijun, Liu, Ruili, Guo, Zhiyi, Luo, Qingquan, Tan, Qiang. 2022. shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8. In Cell death & disease, 13, 685. doi:10.1038/s41419-022-05140-w. https://pubmed.ncbi.nlm.nih.gov/35933405/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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