C57BL/6JCya-Safb2em1flox/Cya
Common Name
Safb2-flox
Product ID
S-CKO-19321
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-224902-Safb2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Safb2-flox Mouse (Catalog S-CKO-19321) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Safb2-flox
Strain ID
CKOCMP-224902-Safb2-B6J-VB
Gene Name
Product ID
S-CKO-19321
Gene Alias
mKIAA0138
Background
C57BL/6JCya
Gene Full Name
scaffold attachment factor B2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 17 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000075510
NCBI Transcript ID
NM_001029979
Target Region
Exon 3~9
Size of Effective Region
~5.5 kb
Overview of Gene Research
Safb2, also known as scaffold attachment factor-B2, is a multifunctional protein. It has been implicated in multiple cellular processes such as chromatin organization, transcriptional regulation, RNA splicing, and stress response [3,4]. It functions as an estrogen receptor corepressor and is thought to have potential tumor-suppressing properties [3,4,5]. It also plays a role in miRNA processing, enabling the processing of suboptimal stem-loop structures in clustered primary miRNA transcripts [2].
SAFB2-null mice were generated to assess its in vivo role [6]. Unlike SAFB1-knockout mice which display high lethality, growth retardation, and infertility in males, SAFB2-null offspring were born at expected ratios and showed no obvious growth or fertility defects. However, SAFB2-null mice had significantly increased testis weight associated with an increased number of Sertoli cells, suggesting a role for SAFB2 in Sertoli cell differentiation and activity [6]. In breast cancer, SAFB2 is downregulated, and its overexpression suppresses cancer cell proliferation, migration, and invasion while promoting apoptosis. It interacts with NFAT5 mRNA, decreasing its stability, and inhibits the Wnt/β-catenin signaling pathway [1].
In conclusion, SAFB2 has diverse functions in the cell, including roles in miRNA processing, transcriptional regulation, and as a potential tumor suppressor. The generation of SAFB2-null mice has revealed its non-redundant functions, especially in Sertoli cell-related processes. In breast cancer, its dysregulation and role in signaling pathways suggest it could be a promising therapeutic target.
References：
1. Zhen, Huifen, Yao, Yarong, Yang, Haibo. 2023. SAFB2 Inhibits the Progression of Breast Cancer by Suppressing the Wnt/β-Catenin Signaling Pathway via NFAT5. In Molecular biotechnology, 65, 1465-1475. doi:10.1007/s12033-022-00649-z. https://pubmed.ncbi.nlm.nih.gov/36652182/
2. Hutter, Katharina, Lohmüller, Michael, Jukic, Almina, Villunger, Andreas, Herzog, Sebastian. . SAFB2 Enables the Processing of Suboptimal Stem-Loop Structures in Clustered Primary miRNA Transcripts. In Molecular cell, 78, 876-889.e6. doi:10.1016/j.molcel.2020.05.011. https://pubmed.ncbi.nlm.nih.gov/32502422/
3. Hong, Elaine A, Gautrey, Hannah L, Elliott, David J, Tyson-Capper, Alison J. . SAFB1- and SAFB2-mediated transcriptional repression: relevance to cancer. In Biochemical Society transactions, 40, 826-30. doi:10.1042/BST20120030. https://pubmed.ncbi.nlm.nih.gov/22817742/
4. Oesterreich, Steffi. . Scaffold attachment factors SAFB1 and SAFB2: Innocent bystanders or critical players in breast tumorigenesis? In Journal of cellular biochemistry, 90, 653-61. doi:. https://pubmed.ncbi.nlm.nih.gov/14587024/
5. Townson, Steven M, Dobrzycka, Klaudia M, Lee, Adrian V, Michaelis, Kai, Oesterreich, Steffi. 2003. SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. In The Journal of biological chemistry, 278, 20059-68. doi:. https://pubmed.ncbi.nlm.nih.gov/12660241/
6. Jiang, Shiming, Katz, Tiffany A, Garee, Jason P, Lee, Adrian V, Oesterreich, Steffi. 2015. Scaffold attachment factor B2 (SAFB2)-null mice reveal non-redundant functions of SAFB2 compared with its paralog, SAFB1. In Disease models & mechanisms, 8, 1121-7. doi:10.1242/dmm.019885. https://pubmed.ncbi.nlm.nih.gov/26092125/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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