C57BL/6JCya-Smarcal1em1flox/Cya
Common Name
Smarcal1-flox
Product ID
S-CKO-19324
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-54380-Smarcal1-B6J-VB
When using this mouse strain in a publication, please cite “Smarcal1-flox Mouse (Catalog S-CKO-19324) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Smarcal1-flox
Strain ID
CKOCMP-54380-Smarcal1-B6J-VB
Gene Name
Product ID
S-CKO-19324
Gene Alias
6030401P21Rik, Mharp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000047615
NCBI RefSeq
NM_018817
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Overview of Gene Research
Smarcal1, an ATP-dependent DNA annealing helicase, belongs to the SNF2-family DNA translocase. It is crucial for maintaining genome stability as it functions in DNA damage repair, especially in reannealing stalled replication forks. It is involved in pathways like cGAS-STING-dependent signaling and also impacts transcriptional regulation [2,3].
In a mouse melanoma model, Smarcal1 loss enhances anti-tumor immune responses. It does so by hindering the ability of tumor cells to induce PD-L1 in response to genomic instability, thus sensitizing tumors to immune checkpoint blockade. This reveals its role in tumor immune evasion [1]. In telomerase-negative glioblastoma cells, Smarcal1 deficiency promotes alternative lengthening of telomeres and tumorigenesis, highlighting its significance in cancer-related processes [4].
In conclusion, Smarcal1 is essential for maintaining genome stability, with key roles in DNA damage repair and transcriptional regulation. Studies using gene knockout models have revealed its importance in tumor immune evasion and tumorigenesis in specific cancer types, providing potential targets for cancer immunotherapy and new insights into cancer mechanisms.
References：
1. Leuzzi, Giuseppe, Vasciaveo, Alessandro, Taglialatela, Angelo, Califano, Andrea, Ciccia, Alberto. 2024. SMARCAL1 is a dual regulator of innate immune signaling and PD-L1 expression that promotes tumor immune evasion. In Cell, 187, 861-881.e32. doi:10.1016/j.cell.2024.01.008. https://pubmed.ncbi.nlm.nih.gov/38301646/
2. Bansal, Ritu, Hussain, Saddam, Chanana, Upasana Bedi, Goel, Isha, Muthuswami, Rohini. 2020. SMARCAL1, the annealing helicase and the transcriptional co-regulator. In IUBMB life, 72, 2080-2096. doi:10.1002/iub.2354. https://pubmed.ncbi.nlm.nih.gov/32754981/
3. Wen, Ya Lei, Lü, Ke Nao, Xu, Xiao Kang, Ding, Liang, Pan, Xue Feng. . [SMARCAL1, roles and mechanisms in genome stability maintenance]. In Yi chuan = Hereditas, 41, 1084-1098. doi:10.16288/j.yczz.19-158. https://pubmed.ncbi.nlm.nih.gov/31857280/
4. Liu, Heng, Xu, Cheng, Diplas, Bill H, He, Yiping, Waitkus, Matthew S. . Cancer-associated SMARCAL1 loss-of-function mutations promote alternative lengthening of telomeres and tumorigenesis in telomerase-negative glioblastoma cells. In Neuro-oncology, 25, 1563-1575. doi:10.1093/neuonc/noad022. https://pubmed.ncbi.nlm.nih.gov/36689342/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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