C57BL/6JCya-Vps4aem1flox/Cya
Common Name:
Vps4a-flox
Product ID:
S-CKO-19444
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Vps4a-flox
Strain ID
CKOCMP-116733-Vps4a-B6J-VB
Gene Name
Product ID
S-CKO-19444
Gene Alias
4930589C15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vps4aem1flox/Cya mice (Catalog S-CKO-19444) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034388
NCBI RefSeq
NM_126165
Target Region
Exon 4~5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Vps4a, a member of the large family of AAA+ ATPases, is a crucial component of the endosomal sorting complex required for transport (ESCRT) system. This system drives membrane remodeling in numerous cellular processes such as receptor degradation, cell division, and neural pruning [3]. Vps4a also plays roles in autophagy, lipophagy, and membrane repair, and is thus of great biological importance. Genetic models, especially KO mouse models, have been instrumental in studying its functions.
In cardiomyocyte-specific Vps4a knockout mice, autophagosome accumulation was observed, and the autophagic flux was blocked, leading to hypertrophic cardiomyopathy and early lethality. This indicates that Vps4a contributes to the sealing of autophagosomes in cardiomyocytes and is essential for normal cardiac function [2]. In another study, deletion of Vps4a in H1299 cells had a similar biological effect as treatment with aloperine, an alkaloid that targets Vps4a to inhibit autophagosome-lysosome fusion, thereby suppressing cancer progression [1]. Also, Vps4a-deficient hearts were more susceptible to cell damage during ischemia/reperfusion injury, as Vps4a is involved in plasma membrane repair [4].
In conclusion, Vps4a is essential for processes like autophagy, membrane repair, and lipophagy. Model-based research, especially using Vps4a KO mouse models, has revealed its critical role in diseases such as cardiomyopathy, cancer, and those related to ischemia/reperfusion injury. Understanding Vps4a's functions provides insights into potential therapeutic strategies for these diseases.
References:
1. Guo, Weina, Zhou, Haifeng, Wang, Jingbo, Luo, Shanshan, Hu, Desheng. 2024. Aloperine Suppresses Cancer Progression by Interacting with VPS4A to Inhibit Autophagosome-lysosome Fusion in NSCLC. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2308307. doi:10.1002/advs.202308307. https://pubmed.ncbi.nlm.nih.gov/39166458/
2. Huang, Xiaozhi, Zhang, Jiayin, Wang, Wenyi, Huang, Zhishan, Han, Peidong. 2023. Vps4a Regulates Autophagic Flux to Prevent Hypertrophic Cardiomyopathy. In International journal of molecular sciences, 24, . doi:10.3390/ijms241310800. https://pubmed.ncbi.nlm.nih.gov/37445978/
3. Dvilansky, Inbar, Altaras, Yarin, Kamenetsky, Nikita, Nachmias, Dikla, Elia, Natalie. 2024. The human AAA-ATPase VPS4A isoform and its co-factor VTA1 have a unique function in regulating mammalian cytokinesis abscission. In PLoS biology, 22, e3002327. doi:10.1371/journal.pbio.3002327. https://pubmed.ncbi.nlm.nih.gov/38687820/
4. Huang, Xiaozhi, Zhang, Jiayin, Xu, Chen, Huang, Zhishan, Han, Peidong. 2025. Vps4a Mediates a Unified Membrane Repair Machinery to Attenuate Ischemia/Reperfusion Injury. In Circulation research, 136, 279-296. doi:10.1161/CIRCRESAHA.124.325290. https://pubmed.ncbi.nlm.nih.gov/39764631/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen