C57BL/6JCya-Ufc1em1flox/Cya
Common Name
Ufc1-flox
Product ID
S-CKO-19469
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-66155-Ufc1-B6J-VB
When using this mouse strain in a publication, please cite “Ufc1-flox Mouse (Catalog S-CKO-19469) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ufc1-flox
Strain ID
CKOCMP-66155-Ufc1-B6J-VB
Gene Name
Product ID
S-CKO-19469
Gene Alias
1110021H02Rik, ESTM29
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000080001
NCBI RefSeq
NM_025388
Target Region
Exon 3~5
Size of Effective Region
~1.7 kb
Overview of Gene Research
Ufc1, short for ubiquitin-fold modifier conjugating enzyme 1, is involved in the ufmylation process, a post-translational modification crucial for various cellular processes such as DNA damage response, protein translation, and ER homeostasis. Ufc1 functions as an E2 enzyme in the three-enzyme cascade (E1-E2-E3) required for UFM1 attachment to target proteins [1,2].
In cancer research, lncRNA UFC1 has been found to act as an oncogene. In non-small-cell lung cancer (NSCLC), its up-regulation promotes tumor progression by binding to EZH2 and epigenetically silencing PTEN expression [3]. In renal cell carcinoma, it stimulates EZH2-induced inhibition of APC expression, aggravating carcinogenesis [4]. Also, serum lncRNA-UFC1 might serve as a biomarker for the diagnosis and prognosis of pancreatic cancer [5]. In addition, knockdown of linc-UFC1 suppresses proliferation and induces apoptosis in colorectal cancer, and lncRNA UFC1 promotes proliferation and migration in breast cancer via the miR-34a/CXCL10 axis [6,7].
In conclusion, Ufc1 plays essential roles in both normal cellular ufmylation processes and in cancer development. The findings from studies on lncRNA UFC1 in different cancers highlight its potential as a diagnostic biomarker and therapeutic target, contributing to a better understanding of cancer mechanisms.
References：
1. Banerjee, Sayanika, Varga, Julia K, Kumar, Manoj, Schueler-Furman, Ora, Wiener, Reuven. 2023. Structural study of UFL1-UFC1 interaction uncovers the role of UFL1 N-terminal helix in ufmylation. In EMBO reports, 24, e56920. doi:10.15252/embr.202356920. https://pubmed.ncbi.nlm.nih.gov/37988244/
2. Kumar, Manoj, Padala, Prasanth, Fahoum, Jamal, Schueler-Furman, Ora, Wiener, Reuven. 2021. Structural basis for UFM1 transfer from UBA5 to UFC1. In Nature communications, 12, 5708. doi:10.1038/s41467-021-25994-6. https://pubmed.ncbi.nlm.nih.gov/34588452/
3. Zang, Xueyan, Gu, Jianmei, Zhang, Jiayin, Xu, Wenrong, Zhang, Xu. 2020. Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression. In Cell death & disease, 11, 215. doi:10.1038/s41419-020-2409-0. https://pubmed.ncbi.nlm.nih.gov/32242003/
4. Wang, Jinteng, Liu, Guanlin. 2023. Long noncoding RNA UFC1 acts as an oncogene via stimulating EZH2-induced inhibition of APC expression in renal cell carcinoma. In Cellular and molecular biology (Noisy-le-Grand, France), 69, 152-156. doi:10.14715/cmb/2023.69.4.24. https://pubmed.ncbi.nlm.nih.gov/37329532/
5. Liu, Peng, Sun, Quan-Quan, Liu, Tong-Xin, Zhu, Yuan, Chen, Ming. 2019. Serum lncRNA-UFC1 as a potential biomarker for diagnosis and prognosis of pancreatic cancer. In International journal of clinical and experimental pathology, 12, 4125-4129. doi:. https://pubmed.ncbi.nlm.nih.gov/31933809/
6. Yu, T, Shan, T-D, Li, J-Y, Zhong, W, Chen, Q-K. 2016. Knockdown of linc-UFC1 suppresses proliferation and induces apoptosis of colorectal cancer. In Cell death & disease, 7, e2228. doi:10.1038/cddis.2016.124. https://pubmed.ncbi.nlm.nih.gov/27195675/
7. Xie, Ruilian, Wang, Maoyuan, Zhou, Wenting, Shi, Huaqiu, Wu, Longqiu. 2019. Long Non-Coding RNA (LncRNA) UFC1/miR-34a Contributes to Proliferation and Migration in Breast Cancer. In Medical science monitor : international medical journal of experimental and clinical research, 25, 7149-7157. doi:10.12659/MSM.917562. https://pubmed.ncbi.nlm.nih.gov/31544897/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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