C57BL/6JCya-Dscc1em1flox/Cya
Common Name:
Dscc1-flox
Product ID:
S-CKO-19474
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dscc1-flox
Strain ID
CKOCMP-72107-Dscc1-B6J-VB
Gene Name
Product ID
S-CKO-19474
Gene Alias
2010006I05Rik; 2600005O03Rik; Dcc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dscc1em1flox/Cya mice (Catalog S-CKO-19474) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110231
NCBI RefSeq
NM_001355594
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Dscc1, also known as DNA replication and sister chromatid cohesion 1, is involved in DNA replication, repair, and sister chromatid cohesion. It functions in a pathway related to these processes and is crucial for maintaining genomic stability [5]. Genetic models, like mouse mutant lines, are valuable for studying Dscc1's function.
In mouse mutant lines, null mutation of Dscc1 led to a significant increase in micronucleus (MN) formation, which are hallmarks of genomic instability. Mice with Dscc1 null mutation also displayed phenotypes characteristic of patients with cohesinopathy disorders, indicating Dscc1's role in maintaining genomic stability in vivo [1].
In human cells, loss of SIRT1 could rescue phenotypes associated with DSCC1 loss, paralleling the restoration of protein acetylation of SMC3 [1]. Additionally, in various cancer studies, DSCC1 was found to be overexpressed in cancers such as lung adenocarcinoma, gastric cancer, and breast cancer, promoting cancer cell proliferation, stemness, EMT, and metastatic potential, and its high expression was often correlated with poor prognosis [2,3,4,6].
In conclusion, Dscc1 is essential for maintaining genomic stability through its role in DNA-related processes. Mouse models with Dscc1 knockout have revealed its significance in conditions related to genomic instability, like cohesinopathy disorders. In cancer research, its overexpression in multiple cancer types suggests it could be a potential biomarker and therapeutic target for these malignancies.
References:
1. Adams, D J, Barlas, B, McIntyre, R E, Jackson, S P, Balmus, G. 2024. Genetic determinants of micronucleus formation in vivo. In Nature, 627, 130-136. doi:10.1038/s41586-023-07009-0. https://pubmed.ncbi.nlm.nih.gov/38355793/
2. Lin, Xu, Liu, Ye-Han, Zhang, Huan-Qi, Li, Yang-Ling, Hu, Jian. 2023. DSCC1 interacts with HSP90AB1 and promotes the progression of lung adenocarcinoma via regulating ER stress. In Cancer cell international, 23, 208. doi:10.1186/s12935-023-03047-w. https://pubmed.ncbi.nlm.nih.gov/37742009/
3. Chang, Sisi, Zhu, Yahui, Xi, Yutan, Ma, Chunzheng, Li, Honglin. 2021. High DSCC1 Level Predicts Poor Prognosis of Lung Adenocarcinoma. In International journal of general medicine, 14, 6961-6974. doi:10.2147/IJGM.S329482. https://pubmed.ncbi.nlm.nih.gov/34707388/
4. Hou, Shiyang, Zhang, Jie, Chi, Xiaoqian, Kang, Chunbo, Shan, Haifeng. . Roles of DSCC1 and GINS1 in gastric cancer. In Medicine, 102, e35681. doi:10.1097/MD.0000000000035681. https://pubmed.ncbi.nlm.nih.gov/37904396/
5. van Schie, Janne Jm, de Lint, Klaas, Pai, Govind M, Wolthuis, Rob Mf, de Lange, Job. 2022. MMS22L-TONSL functions in sister chromatid cohesion in a pathway parallel to DSCC1-RFC. In Life science alliance, 6, . doi:10.26508/lsa.202201596. https://pubmed.ncbi.nlm.nih.gov/36622344/
6. Aljohani, Abrar I. 2024. Prognostic Significance of DSCC1, a Biomarker Associated with Aggressive Features of Breast Cancer. In Medicina (Kaunas, Lithuania), 60, . doi:10.3390/medicina60121929. https://pubmed.ncbi.nlm.nih.gov/39768811/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen