C57BL/6JCya-Snx32em1flox/Cya
Common Name:
Snx32-flox
Product ID:
S-CKO-19478
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Snx32-flox
Strain ID
CKOCMP-225861-Snx32-B6J-VB
Gene Name
Product ID
S-CKO-19478
Gene Alias
B930037P14Rik; Snx6b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snx32em1flox/Cya mice (Catalog S-CKO-19478) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070172
NCBI RefSeq
NM_001024560
Target Region
Exon 2~13
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Snx32 is a member of the sorting nexin (SNX)-BAR family, containing Phox (PX) and Bin/Amphiphysin/Rvs (BAR) domains. It plays significant roles in sorting and membrane trafficking of endosomal cargoes, being involved in pathways like the lysosomal degradation pathway [1].
Silencing of Snx32 leads to defects in intracellular trafficking of the transferrin receptor (TfR) and Cation-Independent Mannose-6-Phosphate Receptor (CIMPR), as well as in neuronal differentiation [2]. In the context of African swine fever virus (ASFV) infection, silencing Snx32 promotes ASFV growth and replication, while overexpressing it has the opposite effect, with Snx32 degrading the ASFV CP204L protein via the RAB1B-dependent autophagy pathway [3]. Additionally, genetic association studies suggest Snx32 may be related to Alzheimer's disease, with its protein abundance potentially having a causal role in the disease [4,5]. In lung squamous cell carcinoma, a 6-gene signature including Snx32 was identified to construct a prognostic RiskScore survival model associated with chronic obstructive pulmonary disease (COPD), with higher RiskScore samples having a worse prognosis [6].
In summary, Snx32 is crucial for endosomal cargo sorting and trafficking. Its functional studies, especially through loss-of-function experiments, have revealed its importance in various biological processes and disease conditions such as neuronal differentiation, ASFV infection, Alzheimer's disease, and lung squamous cell carcinoma prognosis. Understanding Snx32 may provide insights into developing strategies for treating related diseases.
References:
1. Wang, Dou, Zhao, Xia, Wang, Panpan, Liu, Jia-Jia. . SNX32 Regulates Sorting and Trafficking of Activated EGFR to the Lysosomal Degradation Pathway. In Traffic (Copenhagen, Denmark), 25, e12952. doi:10.1111/tra.12952. https://pubmed.ncbi.nlm.nih.gov/39073202/
2. Sugatha, Jini, Priya, Amulya, Raj, Prateek, Pucadyil, Thomas John, Datta, Sunando. 2023. Insights into cargo sorting by SNX32 and its role in neurite outgrowth. In eLife, 12, . doi:10.7554/eLife.84396. https://pubmed.ncbi.nlm.nih.gov/37158588/
3. Yang, Wenping, Li, Lingxia, Zhang, Jing, Li, Dan, Zheng, Haixue. 2024. SNX32 is a host restriction factor that degrades African swine fever virus CP204L via the RAB1B-dependent autophagy pathway. In Journal of virology, 98, e0159923. doi:10.1128/jvi.01599-23. https://pubmed.ncbi.nlm.nih.gov/38169281/
4. Ou, Ya-Nan, Yang, Yu-Xiang, Deng, Yue-Ting, Tan, Lan, Yu, Jin-Tai. 2021. Identification of novel drug targets for Alzheimer's disease by integrating genetics and proteomes from brain and blood. In Molecular psychiatry, 26, 6065-6073. doi:10.1038/s41380-021-01251-6. https://pubmed.ncbi.nlm.nih.gov/34381170/
5. Kibinge, Nelson K, Relton, Caroline L, Gaunt, Tom R, Richardson, Tom G. 2020. Characterizing the Causal Pathway for Genetic Variants Associated with Neurological Phenotypes Using Human Brain-Derived Proteome Data. In American journal of human genetics, 106, 885-892. doi:10.1016/j.ajhg.2020.04.007. https://pubmed.ncbi.nlm.nih.gov/32413284/
6. Li, Zheng, Xu, Dan, Jing, Jing, Jiang, Min, Li, Fengsen. 2022. Identification and Validation of Prognostic Markers for Lung Squamous Cell Carcinoma Associated with Chronic Obstructive Pulmonary Disease. In Journal of oncology, 2022, 4254195. doi:10.1155/2022/4254195. https://pubmed.ncbi.nlm.nih.gov/36035311/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen