C57BL/6JCya-Slc50a1em1flox/Cya
Common Name:
Slc50a1-flox
Product ID:
S-CKO-19490
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc50a1-flox
Strain ID
CKOCMP-19729-Slc50a1-B6J-VB
Gene Name
Product ID
S-CKO-19490
Gene Alias
MmSWEET1; Rag1ap1; Rga
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc50a1em1flox/Cya mice (Catalog S-CKO-19490) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029565
NCBI RefSeq
NM_009057
Target Region
Exon 1~6
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Slc50a1, a member of the SLC family, is a novel sugar transporter and membrane protein. As part of the SLC50 family, which in humans has only one member, it may be involved in glucose transmembrane transport [3,5].
In hepatocellular carcinoma (HCC), Slc50a1 is significantly upregulated, correlating with unfavorable prognosis. It can regulate cellular glycolysis and the cell cycle, promoting HCC cell proliferation and reducing apoptosis, while enhancing resistance to drugs like doxorubicin (DOX) and 2-DG. The m6A methyltransferase METTL3 mediates its methylation modification, and IGF2BP2 promotes its stability and translational expression [1].
In early breast cancer, Slc50a1 serum levels are higher compared to those with benign breast disease and healthy controls, and its upregulation may be associated with unfavorable prognosis, suggesting it could be a potential diagnostic biomarker [2]. Bioinformatics analysis also confirmed its diagnostic value in breast cancer, and its protein expression was associated with estrogen receptor and HER2 status, while high-grade breast cancer patients with high Slc50a1 levels had unfavorable 3-year outcomes, indicating its potential as an independent prognostic factor [3].
Genome-wide association studies found that a locus near Slc50a1 is associated with 1,5-anhydroglucitol concentrations, suggesting a role in glucose metabolism [4].
Whole-exome sequencing in Chinese early-onset Parkinson's disease (EOPD) patients identified rare variants in Slc50a1, though the significance of these variants is yet to be fully understood [6].
In Pakistani families with non-syndromic intellectual disability, a novel homozygous variant in Slc50a1 was found, suggesting its potential role in cognitive impairment [7].
In cutaneous T-cell lymphomas, an alternative splicing event related to Slc50a1 was identified in association with histone deacetylase inhibitor resistance or sensitivity [8].
In summary, Slc50a1 is involved in multiple biological processes and diseases. Its role in glucose-related metabolism and as a potential biomarker in cancers, along with its possible connections to neurodegenerative and cognitive disorders, highlights its importance. Research on Slc50a1 using various genetic models could further clarify its functions and provide insights into disease mechanisms and potential therapeutic targets.
References:
1. Wang, Ganggang, Jin, Wenzhi, Zhang, Lianmei, Zhou, Zhijie, Wang, Xiaoliang. 2024. SLC50A1 inhibits the doxorubicin sensitivity in hepatocellular carcinoma cells through regulating the tumor glycolysis. In Cell death discovery, 10, 495. doi:10.1038/s41420-024-02261-3. https://pubmed.ncbi.nlm.nih.gov/39695152/
2. Zhang, Qunchen, Fang, Yutong, She, Chuanghong, Chen, Chunfa, Wu, Jundong. 2022. Diagnostic and prognostic significance of SLC50A1 expression in patients with primary early breast cancer. In Experimental and therapeutic medicine, 24, 616. doi:10.3892/etm.2022.11553. https://pubmed.ncbi.nlm.nih.gov/36160901/
3. Wang, Yu, Shu, Yao, Gu, Congyang, Fan, Yu. 2019. The novel sugar transporter SLC50A1 as a potential serum-based diagnostic and prognostic biomarker for breast cancer. In Cancer management and research, 11, 865-876. doi:10.2147/CMAR.S190591. https://pubmed.ncbi.nlm.nih.gov/30697078/
4. Li, Man, Maruthur, Nisa M, Loomis, Stephanie J, Selvin, Elizabeth, Köttgen, Anna. 2017. Genome-wide association study of 1,5-anhydroglucitol identifies novel genetic loci linked to glucose metabolism. In Scientific reports, 7, 2812. doi:10.1038/s41598-017-02287-x. https://pubmed.ncbi.nlm.nih.gov/28588231/
5. Wright, Ernest M. . Glucose transport families SLC5 and SLC50. In Molecular aspects of medicine, 34, 183-96. doi:10.1016/j.mam.2012.11.002. https://pubmed.ncbi.nlm.nih.gov/23506865/
6. Li, ChunYu, Ou, RuWei, Chen, YongPing, Wu, Ying, Shang, HuiFang. 2021. Mutation analysis of seven SLC family transporters for early-onset Parkinson's disease in Chinese population. In Neurobiology of aging, 103, 152.e1-152.e6. doi:10.1016/j.neurobiolaging.2021.02.022. https://pubmed.ncbi.nlm.nih.gov/33781609/
7. Ahmed, Iftikhar, Muzammal, Muhammad, Khan, Muzammil Ahmad, Alam, Khurshid, Mir, Asif. 2023. Identification of Four Novel Candidate Genes for Non-syndromic Intellectual Disability in Pakistani Families. In Biochemical genetics, 62, 2571-2586. doi:10.1007/s10528-023-10556-w. https://pubmed.ncbi.nlm.nih.gov/37985543/
8. Yu, Shirong, Zhang, Jingzhan, Ding, Yuan, Kang, Xiaojing, Pu, Xiongming. 2022. Genome-wide identification of alternative splicing associated with histone deacetylase inhibitor in cutaneous T-cell lymphomas. In Frontiers in genetics, 13, 937623. doi:10.3389/fgene.2022.937623. https://pubmed.ncbi.nlm.nih.gov/36147491/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen