C57BL/6JCya-Apohem1flox/Cya
Common Name
Apoh-flox
Product ID
S-CKO-19497
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-11818-Apoh-B6J-VB
Status
When using this mouse strain in a publication, please cite “Apoh-flox Mouse (Catalog S-CKO-19497) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Apoh-flox
Strain ID
CKOCMP-11818-Apoh-B6J-VB
Gene Name
Product ID
S-CKO-19497
Gene Alias
B2GPI, beta2-GPI, beta-2-GPI
Background
C57BL/6JCya
Gene Full Name
apolipoprotein H
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000000049
NCBI Transcript ID
NM_013475
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Overview of Gene Research
Apoh, also known as apolipoprotein H, is involved in lipid metabolism, regulating triglycerides, and has a role in pathways related to complement and coagulation regulation [3,6,7]. It is one of the apolipoproteins that can bind free fatty acids, regulate the activity of many proteins involved in HDL metabolism, inhibit lipid transfer, and control the endogenous coagulation cascade [5].
In antiphospholipid syndrome (APS), both exosomes containing APOH (APOH-exos) and APS-exos impair vascular development and lead to pregnancy complications. APOH-exos may be key in the pathogenesis of APS as they can directly enter human umbilical vein endothelial cells (HUVECs) and may act through the phospho-extracellular signal-regulated kinase pathway [1]. Also, in APS patients, polymorphisms in the APOH gene (rs1801690, rs52797880, and rs8178847) are associated with susceptibility to recurrent pregnancy loss (RPL) [2].
In type 2 diabetic patients, APOH plasma levels are associated with metabolic syndrome (MS) alterations and vascular disease, with enhanced levels due to increased liver synthesis [3]. In diabetic kidney disease, the mRNA expression level of Apoh was declined [4]. In Influenza infection, low plasma levels of ApoH are associated with a worse prognosis and respiratory failure [7].
In conclusion, Apoh is crucial in lipid metabolism, complement and coagulation regulation. Its study through various models has revealed its significant roles in diseases such as APS, type 2 diabetes, diabetic kidney disease, and influenza infection. Understanding Apoh's functions can potentially provide new therapeutic targets for these diseases.
References：
1. Tan, Yuan, Bian, Yiding, Song, Yunfeng, Zhang, Qinhua, Wan, Xiaoping. 2021. Exosome-Contained APOH Associated With Antiphospholipid Syndrome. In Frontiers in immunology, 12, 604222. doi:10.3389/fimmu.2021.604222. https://pubmed.ncbi.nlm.nih.gov/34040601/
2. Deng, Xujing, Sang, Qing, Zhang, Ruixiu, Mu, Jian, Bao, Shihua. 2023. The association of APOH and NCF1 polymorphisms on susceptibility to recurrent pregnancy loss in women with antiphospholipid syndrome. In Journal of assisted reproduction and genetics, 40, 1703-1712. doi:10.1007/s10815-023-02829-5. https://pubmed.ncbi.nlm.nih.gov/37243946/
3. Castro, Antoni, Lázaro, Iolanda, Selva, David M, Simó, Rafael, Masana, Lluís. 2009. APOH is increased in the plasma and liver of type 2 diabetic patients with metabolic syndrome. In Atherosclerosis, 209, 201-5. doi:10.1016/j.atherosclerosis.2009.09.072. https://pubmed.ncbi.nlm.nih.gov/19878946/
4. Zhao, Jing, He, Kaiying, Du, Hongxuan, Zhou, Xiaochun, Wang, Jianqin. 2022. Bioinformatics prediction and experimental verification of key biomarkers for diabetic kidney disease based on transcriptome sequencing in mice. In PeerJ, 10, e13932. doi:10.7717/peerj.13932. https://pubmed.ncbi.nlm.nih.gov/36157062/
5. Deng, Siying, Xu, Yangkai, Zheng, Lemin. . HDL Structure. In Advances in experimental medicine and biology, 1377, 1-11. doi:10.1007/978-981-19-1592-5_1. https://pubmed.ncbi.nlm.nih.gov/35575917/
6. Gu, W, Ray, K, Pearce-Kelling, S, Acland, G M, Aguirre, G D. . Evaluation of the APOH gene as a positional candidate for prcd in dogs. In Investigative ophthalmology & visual science, 40, 1229-37. doi:. https://pubmed.ncbi.nlm.nih.gov/10235557/
7. Garcinuño, Sara, Lalueza, Antonio, Gil-Etayo, Francisco Javier, Serrano, Antonio, Serrano, Manuel. 2024. Immune dysregulation is an important factor in the underlying complications in Influenza infection. ApoH, IL-8 and IL-15 as markers of prognosis. In Frontiers in immunology, 15, 1443096. doi:10.3389/fimmu.2024.1443096. https://pubmed.ncbi.nlm.nih.gov/39176097/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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