C57BL/6JCya-Apohem1flox/Cya
Common Name:
Apoh-flox
Product ID:
S-CKO-19497
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Apoh-flox
Strain ID
CKOCMP-11818-Apoh-B6J-VB
Gene Name
Product ID
S-CKO-19497
Gene Alias
B2GPI; beta-2-GPI; beta2-GPI
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Apohem1flox/Cya mice (Catalog S-CKO-19497) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000000049
NCBI RefSeq
NM_013475
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Apoh, also known as apolipoprotein H, is involved in lipid metabolism, regulating triglycerides, and has a role in pathways related to complement and coagulation regulation [3,6,7]. It is one of the apolipoproteins that can bind free fatty acids, regulate the activity of many proteins involved in HDL metabolism, inhibit lipid transfer, and control the endogenous coagulation cascade [5].
In antiphospholipid syndrome (APS), both exosomes containing APOH (APOH-exos) and APS-exos impair vascular development and lead to pregnancy complications. APOH-exos may be key in the pathogenesis of APS as they can directly enter human umbilical vein endothelial cells (HUVECs) and may act through the phospho-extracellular signal-regulated kinase pathway [1]. Also, in APS patients, polymorphisms in the APOH gene (rs1801690, rs52797880, and rs8178847) are associated with susceptibility to recurrent pregnancy loss (RPL) [2].
In type 2 diabetic patients, APOH plasma levels are associated with metabolic syndrome (MS) alterations and vascular disease, with enhanced levels due to increased liver synthesis [3]. In diabetic kidney disease, the mRNA expression level of Apoh was declined [4]. In Influenza infection, low plasma levels of ApoH are associated with a worse prognosis and respiratory failure [7].
In conclusion, Apoh is crucial in lipid metabolism, complement and coagulation regulation. Its study through various models has revealed its significant roles in diseases such as APS, type 2 diabetes, diabetic kidney disease, and influenza infection. Understanding Apoh's functions can potentially provide new therapeutic targets for these diseases.
References:
1. Tan, Yuan, Bian, Yiding, Song, Yunfeng, Zhang, Qinhua, Wan, Xiaoping. 2021. Exosome-Contained APOH Associated With Antiphospholipid Syndrome. In Frontiers in immunology, 12, 604222. doi:10.3389/fimmu.2021.604222. https://pubmed.ncbi.nlm.nih.gov/34040601/
2. Deng, Xujing, Sang, Qing, Zhang, Ruixiu, Mu, Jian, Bao, Shihua. 2023. The association of APOH and NCF1 polymorphisms on susceptibility to recurrent pregnancy loss in women with antiphospholipid syndrome. In Journal of assisted reproduction and genetics, 40, 1703-1712. doi:10.1007/s10815-023-02829-5. https://pubmed.ncbi.nlm.nih.gov/37243946/
3. Castro, Antoni, Lázaro, Iolanda, Selva, David M, Simó, Rafael, Masana, Lluís. 2009. APOH is increased in the plasma and liver of type 2 diabetic patients with metabolic syndrome. In Atherosclerosis, 209, 201-5. doi:10.1016/j.atherosclerosis.2009.09.072. https://pubmed.ncbi.nlm.nih.gov/19878946/
4. Zhao, Jing, He, Kaiying, Du, Hongxuan, Zhou, Xiaochun, Wang, Jianqin. 2022. Bioinformatics prediction and experimental verification of key biomarkers for diabetic kidney disease based on transcriptome sequencing in mice. In PeerJ, 10, e13932. doi:10.7717/peerj.13932. https://pubmed.ncbi.nlm.nih.gov/36157062/
5. Deng, Siying, Xu, Yangkai, Zheng, Lemin. . HDL Structure. In Advances in experimental medicine and biology, 1377, 1-11. doi:10.1007/978-981-19-1592-5_1. https://pubmed.ncbi.nlm.nih.gov/35575917/
6. Gu, W, Ray, K, Pearce-Kelling, S, Acland, G M, Aguirre, G D. . Evaluation of the APOH gene as a positional candidate for prcd in dogs. In Investigative ophthalmology & visual science, 40, 1229-37. doi:. https://pubmed.ncbi.nlm.nih.gov/10235557/
7. Garcinuño, Sara, Lalueza, Antonio, Gil-Etayo, Francisco Javier, Serrano, Antonio, Serrano, Manuel. 2024. Immune dysregulation is an important factor in the underlying complications in Influenza infection. ApoH, IL-8 and IL-15 as markers of prognosis. In Frontiers in immunology, 15, 1443096. doi:10.3389/fimmu.2024.1443096. https://pubmed.ncbi.nlm.nih.gov/39176097/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen