C57BL/6JCya-Zfp683em1flox/Cya
Common Name
Zfp683-flox
Product ID
S-CKO-19534
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-100503878-Zfp683-B6J-VA
Status
When using this mouse strain in a publication, please cite “Zfp683-flox Mouse (Catalog S-CKO-19534) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Zfp683-flox
Strain ID
CKOCMP-100503878-Zfp683-B6J-VA
Gene Name
Zfp683
Product ID
S-CKO-19534
Gene Alias
Gm13060, Hobit
Background
C57BL/6JCya
Gene Full Name
--
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 4 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000105884
NCBI Transcript ID
--
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Overview of Gene Research
Zfp683, also known as ZNF683 in humans, encodes the transcription factor Hobit. It is crucial for the tissue residency of certain lymphocytes. It is involved in the development of tissue-resident memory T (Trm) cells, natural killer T (NKT) cells, and liver-resident NK cells, and plays a role in the transcriptional program that enforces tissue retention of these cells [1].
In Zfp683 reporter mice, the correlation of Hobit expression with type 1 innate lymphoid cells (ILC1s) is tissue-and context-dependent. Zfp683 deletion led to a marked decrease in granzyme-and interferon-gamma (IFNγ)-producing ILC1s in the liver, slightly fewer ILC1s and more Eomes+ TCF1+ ILC1-like NK cells in salivary glands, and only reduced production of granzyme B by ILC1 in the intestinal mucosa. Ablation of Zfp683-dependent liver ILC1 led to increased viral load in the presence of intact adaptive and innate immune cells critical for mouse cytomegalovirus (MCMV) clearance [2,3].
In conclusion, Zfp683 is central to the tissue-resident lymphocyte transcriptional program, affecting ILC1 numbers and function in different tissues. Studies using Zfp683-related mouse models have revealed its importance in antiviral immunity, suggesting it may be a potential target for understanding and treating viral-related diseases.
References：
1. Mackay, Laura K, Minnich, Martina, Kragten, Natasja A M, Kallies, Axel, van Gisbergen, Klaas P J M. . Hobit and Blimp1 instruct a universal transcriptional program of tissue residency in lymphocytes. In Science (New York, N.Y.), 352, 459-63. doi:10.1126/science.aad2035. https://pubmed.ncbi.nlm.nih.gov/27102484/
2. Yomogida, Kentaro, Bigley, Tarin M, Trsan, Tihana, Egawa, Takeshi, Colonna, Marco. . Hobit confers tissue-dependent programs to type 1 innate lymphoid cells. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2117965118. https://pubmed.ncbi.nlm.nih.gov/34880136/
3. Weizman, Orr-El, Adams, Nicholas M, Schuster, Iona S, Sun, Joseph C, O'Sullivan, Timothy E. 2017. ILC1 Confer Early Host Protection at Initial Sites of Viral Infection. In Cell, 171, 795-808.e12. doi:10.1016/j.cell.2017.09.052. https://pubmed.ncbi.nlm.nih.gov/29056343/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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