C57BL/6JCya-Rac3em1flox/Cya
Common Name
Rac3-flox
Product ID
S-CKO-19540
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-170758-Rac3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Rac3-flox Mouse (Catalog S-CKO-19540) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Rac3-flox
Strain ID
CKOCMP-170758-Rac3-B6J-VA
Gene Name
Product ID
S-CKO-19540
Gene Alias
Rac1B
Background
C57BL/6JCya
Gene Full Name
Rac family small GTPase 3
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000018156
NCBI Transcript ID
NM_133223
Target Region
Exon 2~6
Size of Effective Region
~2.4 kb
Overview of Gene Research
Rac3, short for Ras-related C3 botulinum toxin substrate 3, is a member of the Rho family small guanosine triphosphatases (GTPases). It is crucial in regulating the cytoskeleton, playing significant roles in cellular and developmental biology, as well as in pathological processes such as neurodevelopmental disorders and cancer [1].
In mouse models, Rac1 and Rac3 double knockout (Rac1/Rac3-DKO) mice under the control of the Atoh1 promoter showed normal cochlear hair cell (HC) morphology at 13 weeks and normal hearing function at 24 weeks, indicating that Rac3 is dispensable for the maturation of cochlear HCs in the post-mitotic state or for hearing maintenance following HC maturation [5]. In bladder cancer, knockdown of Rac3 inhibited cell proliferation, migration, and invasion, and caused cell cycle arrest. It activated PI3K/AKT/mTOR-mediated autophagy, suggesting a potential therapeutic strategy by targeting Rac3 and autophagy simultaneously [2]. In endometrial cancer, Rac3 promoted cell proliferation and invasion by increasing fatty acid synthase (FASN) expression, and its high expression was correlated with poor prognosis [4]. Also, in endothelial cells, Rac3 promoted ox-LDL induced endothelial dysfunction by down-regulating autophagy [3].
In conclusion, Rac3 is involved in various biological processes and disease conditions. Mouse knockout models have been valuable in revealing its dispensable role in cochlear HC maturation and hearing maintenance. In cancer, such as bladder and endometrial cancer, Rac3 promotes tumor progression, highlighting its potential as a therapeutic target. Its role in endothelial cell dysfunction also indicates its importance in atherosclerosis-related pathologies.
References：
1. de Curtis, Ivan. 2019. The Rac3 GTPase in Neuronal Development, Neurodevelopmental Disorders, and Cancer. In Cells, 8, . doi:10.3390/cells8091063. https://pubmed.ncbi.nlm.nih.gov/31514269/
2. Wang, Liwei, Shi, Jiazhong, Liu, Sha, Yang, Jin, Chen, Zhiwen. 2022. RAC3 Inhibition Induces Autophagy to Impair Metastasis in Bladder Cancer Cells via the PI3K/AKT/mTOR Pathway. In Frontiers in oncology, 12, 915240. doi:10.3389/fonc.2022.915240. https://pubmed.ncbi.nlm.nih.gov/35847878/
3. He, Dan, Xu, Ling, Wu, Yuhang, Wang, Hongyan, Qu, Peng. 2019. Rac3, but not Rac1, promotes ox-LDL induced endothelial dysfunction by downregulating autophagy. In Journal of cellular physiology, 235, 1531-1542. doi:10.1002/jcp.29072. https://pubmed.ncbi.nlm.nih.gov/31332791/
4. Meijuan, Cai, Fang, Liu, Min, Fang, Qian, Wang. 2022. Hypomethylated gene RAC3 induces cell proliferation and invasion by increasing FASN expression in endometrial cancer. In The international journal of biochemistry & cell biology, 150, 106274. doi:10.1016/j.biocel.2022.106274. https://pubmed.ncbi.nlm.nih.gov/35917927/
5. Nakamura, Takashi, Sakaguchi, Hirofumi, Mohri, Hiroaki, Saito, Naoaki, Ueyama, Takehiko. 2023. Dispensable role of Rac1 and Rac3 after cochlear hair cell specification. In Journal of molecular medicine (Berlin, Germany), 101, 843-854. doi:10.1007/s00109-023-02317-4. https://pubmed.ncbi.nlm.nih.gov/37204479/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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