C57BL/6NCya-Cpeb2em1flox/Cya
Common Name:
Cpeb2-flox
Product ID:
S-CKO-19784
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cpeb2-flox
Strain ID
CKOCMP-231207-Cpeb2-B6N-VA
Gene Name
Product ID
S-CKO-19784
Gene Alias
A630055H10Rik; Cpe-bp2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cpeb2em1flox/Cya mice (Catalog S-CKO-19784) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169035
NCBI RefSeq
NM_001177379
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Cpeb2, cytoplasmic polyadenylate element-binding protein 2, is an RNA-binding protein that plays crucial roles in multiple biological processes. It is involved in translational regulation, which impacts various cellular functions, and is associated with pathways related to cell proliferation, apoptosis, and differentiation. Genetic models, such as knockout (KO) mouse models, have been instrumental in studying its functions [1-9].
In glioma, Cpeb2 is downregulated and its overexpression inhibits cell proliferation and promotes apoptosis by increasing p21 mRNA stability and inducing G1 cell-cycle arrest [1]. In brown adipocytes, Cpeb2-KO mice show impaired thermogenesis and obesogenic phenotypes as Cpeb2-activated Prdm16 translation is critical for brown adipocyte function [2]. In preeclampsia, Cpeb2 is lowly expressed, and its upregulation promotes trophoblast cell functions by inhibiting SSTR3 translation through polyadenylation [3]. In multiple myeloma, Cpeb2 is upregulated, and its downregulation reduces cell proliferation and angiogenesis [4]. In renal cancer, Cpeb2 is upregulated and promotes cell proliferation and migration via a negative feedback loop with p53 [5].
In conclusion, Cpeb2 has diverse functions in different biological contexts. Model-based research, especially KO mouse models, has revealed its significance in diseases like glioma, obesity, preeclampsia, multiple myeloma, and renal cancer. These findings highlight Cpeb2 as a potential biomarker and therapeutic target in various disease areas.
References:
1. Zhao, Guang, Zhao, Zhongjun, Xia, Mingyi, Li, Xiang, Di, Jiehui. 2023. CPEB2 inhibit cell proliferation through upregulating p21 mRNA stability in glioma. In Scientific reports, 13, 23103. doi:10.1038/s41598-023-50848-0. https://pubmed.ncbi.nlm.nih.gov/38158431/
2. Lu, Wen-Hsin, Chen, Hui-Feng, King, Pei-Chih, Peng, Chi, Huang, Yi-Shuian. 2024. CPEB2-activated Prdm16 translation promotes brown adipocyte function and prevents obesity. In Molecular metabolism, 89, 102034. doi:10.1016/j.molmet.2024.102034. https://pubmed.ncbi.nlm.nih.gov/39305947/
3. Zhao, Yanhua, Zhang, Liran, Yang, Jingjing, Li, Caiwen, Li, Ping. 2024. CPEB2 inhibits preeclampsia progression by regulating SSTR3 translation through polyadenylation. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167191. doi:10.1016/j.bbadis.2024.167191. https://pubmed.ncbi.nlm.nih.gov/38648900/
4. Zeng, Piaorong, Wang, Fujue, Long, Xingxing, Li, Junjun, Luo, Zeyu. 2023. CPEB2 enhances cell growth and angiogenesis by upregulating ARPC5 mRNA stability in multiple myeloma. In Journal of orthopaedic surgery and research, 18, 384. doi:10.1186/s13018-023-03835-0. https://pubmed.ncbi.nlm.nih.gov/37231521/
5. Di, Jiehui, Zhao, Guang, Wang, Hui, Liu, Yong, Hu, Ying. 2021. A p53/CPEB2 negative feedback loop regulates renal cancer cell proliferation and migration. In Journal of genetics and genomics = Yi chuan xue bao, 48, 606-617. doi:10.1016/j.jgg.2021.05.011. https://pubmed.ncbi.nlm.nih.gov/34362680/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen