C57BL/6JCya-Tinagl1em1flox/Cya
Common Name:
Tinagl1-flox
Product ID:
S-CKO-19799
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Tinagl1-flox
Strain ID
CKOCMP-94242-Tinagl1-B6J-VA
Gene Name
Product ID
S-CKO-19799
Gene Alias
1110021J17Rik; AZ-1; AZ1; Arg1; Lcn7; TARP; Tinagl
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tinagl1em1flox/Cya mice (Catalog S-CKO-19799) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105998
NCBI RefSeq
NM_023476
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Tinagl1, also known as Tubulointerstitial nephritis antigen-like 1, is a matricellular protein. It plays roles in cell adhesion, modulating cell proliferation, migration, and differentiation. It is associated with multiple signaling pathways such as integrin/FAK, EGFR, TGF-β, and ERK [1,2,5,8]. It has significance in various biological processes including wound healing, muscle development, and in disease conditions like cancer, Crohn's Disease, and Helicobacter pylori-associated gastritis [1-9].
In triple-negative breast cancer (TNBC), ectopic expression and therapeutic delivery of Tinagl1 suppress TNBC progression and metastasis by directly binding to integrin α5β1, αvβ1, and EGFR, and inhibiting FAK and EGFR signaling pathways [1]. In Crohn's Disease, mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis via SMAD4 [2]. In breast cancer, low TINAGL1 expression is a marker for poor prognosis [3]. In diabetes, down-regulated TINAGL1 in fibroblasts impairs wound healing, while exogenous TINAGL1 promotes wound healing in diabetic mice [4]. In hepatocellular carcinoma, TINAGL1 promotes carcinogenesis and metastasis via the TGF-β/Smad3/VEGF axis [5]. In esophageal cancer, YTHDF1 facilitates cancer progression by augmenting m6A-dependent TINAGL1 translation [6]. In Helicobacter pylori infection, TINAGL1 promotes bacterial colonization and gastritis [7]. In muscle development, Tinagl1-deficient mice show reduced body mass, abnormal myofibers, and decreased capillary density, suggesting Tinagl1 is required for normal muscle and capillary development through ERK signaling activation [8]. In tamoxifen-resistant breast cancer cells, Tinagl1 restores tamoxifen sensitivity by blocking EGFR and β1-integrin/FAK signaling pathways [9].
In conclusion, Tinagl1 is a multifunctional protein involved in various biological processes and disease conditions. Studies using gene-knockout or related models have revealed its crucial roles in cancer metastasis, fibrosis, wound healing, muscle development, and bacterial-associated inflammation. Understanding Tinagl1's functions provides potential therapeutic targets for related diseases.
References:
1. Shen, Minhong, Jiang, Yi-Zhou, Wei, Yong, Shao, Zhi-Ming, Kang, Yibin. 2019. Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling. In Cancer cell, 35, 64-80.e7. doi:10.1016/j.ccell.2018.11.016. https://pubmed.ncbi.nlm.nih.gov/30612941/
2. Chen, Yidong, Li, Junrong, Zhang, Xiaopeng, Li, Jiamin, Zhu, Liangru. 2024. Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4. In Journal of advanced research, 70, 139-158. doi:10.1016/j.jare.2024.05.016. https://pubmed.ncbi.nlm.nih.gov/38750695/
3. Kato, Akiko, Kondo, Naoto, Wanifuchi-Endo, Yumi, Takahashi, Satoru, Toyama, Tatsuya. 2022. Low TINAGL1 expression is a marker for poor prognosis in breast cancer. In Journal of cancer research and clinical oncology, 149, 4771-4782. doi:10.1007/s00432-022-04394-3. https://pubmed.ncbi.nlm.nih.gov/36229542/
4. Tian, Wen-Qing, Chen, Si-Yu, Chuan, Feng-Ning, Zhao, Wen-Rui, Zhou, Bo. . Down-regulated TINAGL1 in fibroblasts impairs wound healing in diabetes. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22235. doi:10.1096/fj.202101438RR. https://pubmed.ncbi.nlm.nih.gov/35199864/
5. Sun, Lu, Dong, Zihui, Gu, Hongli, Guo, Zhixian, Yu, Zujiang. 2019. TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression. In Cancer management and research, 11, 767-775. doi:10.2147/CMAR.S190390. https://pubmed.ncbi.nlm.nih.gov/30697069/
6. Zhang, Lin, Cai, Enmin, Xu, Yuting, Pei, Dongsheng, Wang, Qingling. 2024. YTHDF1 facilitates esophageal cancer progression via augmenting m6A-dependent TINAGL1 translation. In Cellular signalling, 122, 111332. doi:10.1016/j.cellsig.2024.111332. https://pubmed.ncbi.nlm.nih.gov/39098703/
7. Teng, Yongsheng, Xie, Rui, Xu, Jingyu, Zou, Quanming, Zhuang, Yuan. 2023. Tubulointerstitial nephritis antigen-like 1 is a novel matricellular protein that promotes gastric bacterial colonization and gastritis in the setting of Helicobacter pylori infection. In Cellular & molecular immunology, 20, 924-940. doi:10.1038/s41423-023-01055-4. https://pubmed.ncbi.nlm.nih.gov/37336990/
8. Sato, Yoriko, Kawashima, Keisuke, Fukui, Emiko, Yoshizawa, Fumiaki, Sato, Yusuke. 2022. Functional analysis reveals that Tinagl1 is required for normal muscle development in mice through the activation of ERK signaling. In Biochimica et biophysica acta. Molecular cell research, 1869, 119294. doi:10.1016/j.bbamcr.2022.119294. https://pubmed.ncbi.nlm.nih.gov/35597451/
9. Yuan, Jie, Yuan, Li, Yang, Li, Wei, Changsheng, Luo, Chengyu. . Tinagl1 restores tamoxifen sensitivity and blocks fibronectin-induced EMT by simultaneously blocking the EGFR and β1-integrin/FAK signaling pathways in tamoxifen-resistant breast cancer cells. In IUBMB life, 77, e2940. doi:10.1002/iub.2940. https://pubmed.ncbi.nlm.nih.gov/39817673/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen