C57BL/6JCya-Dhx16em1flox/Cya
Common Name:
Dhx16-flox
Product ID:
S-CKO-19821
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dhx16-flox
Strain ID
CKOCMP-69192-Dhx16-B6J-VB
Gene Name
Product ID
S-CKO-19821
Gene Alias
2410006N22Rik; DBP2; Ddx16; mKIAA0577
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dhx16em1flox/Cya mice (Catalog S-CKO-19821) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025292
NCBI RefSeq
NM_026987
Target Region
Exon 8~9
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Dhx16, also known as DBP2, is an ATP-dependent RNA helicase and a key spliceosome component. It plays a vital role in pre-mRNA splicing, a process that increases protein diversity. Through this function, Dhx16 is involved in various biological processes and is essential for the maintenance of normal cellular functions [2,3,4,5].
Using conditional knockout (CKO) mice, it was demonstrated that loss of Dhx16 in the hematopoietic system leads to significant depletion of hematopoietic stem and progenitor cells, bone marrow failure, and rapid mortality. Dhx16-deficient HSCs show impaired quiescence, abnormal ribosome assembly, increased apoptosis, and decreased self-renewal capacity, revealing its critical role in HSC homeostasis through regulation of alternative splicing and ribosome assembly [1].
In conclusion, Dhx16 is crucial for pre-mRNA splicing and ribosome assembly, which are fundamental to normal cellular function. The Dhx16 CKO mouse model has provided key insights into its role in hematopoietic diseases, highlighting its potential as a therapeutic target for ribosomopathies.
References：
1. Li, Zhigang, Fan, Jiankun, Xiao, Yalan, Zhao, Xueya, Hou, Yu. 2024. Essential role of Dhx16-mediated ribosome assembly in maintenance of hematopoietic stem cells. In Leukemia, 38, 2699-2708. doi:10.1038/s41375-024-02423-3. https://pubmed.ncbi.nlm.nih.gov/39333759/
2. Gencheva, Marieta, Kato, Mitsuo, Newo, Alain N S, Lin, Ren-Jang. . Contribution of DEAH-box protein DHX16 in human pre-mRNA splicing. In The Biochemical journal, 429, 25-32. doi:10.1042/BJ20100266. https://pubmed.ncbi.nlm.nih.gov/20423332/
3. Gencheva, Marieta, Lin, Ting-Yu, Wu, Xiwei, Lin, Shwu-Bin, Lin, Ren-Jang. 2010. Nuclear retention of unspliced pre-mRNAs by mutant DHX16/hPRP2, a spliceosomal DEAH-box protein. In The Journal of biological chemistry, 285, 35624-32. doi:10.1074/jbc.M110.122309. https://pubmed.ncbi.nlm.nih.gov/20841358/
4. Zang, Shengbing, Lin, Ting-Yu, Chen, Xinji, Huang, Aimin, Lin, Ren-Jang. 2014. GPKOW is essential for pre-mRNA splicing in vitro and suppresses splicing defect caused by dominant-negative DHX16 mutation in vivo. In Bioscience reports, 34, e00163. doi:10.1042/BSR20140142. https://pubmed.ncbi.nlm.nih.gov/25296192/
5. Schmitzová, Jana, Cretu, Constantin, Dienemann, Christian, Urlaub, Henning, Pena, Vladimir. 2023. Structural basis of catalytic activation in human splicing. In Nature, 617, 842-850. doi:10.1038/s41586-023-06049-w. https://pubmed.ncbi.nlm.nih.gov/37165190/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen