C57BL/6JCya-Pcsk9em1/Cya
Common Name
Pcsk9-KO
Product ID
S-KO-00067
Backgroud
C57BL/6JCya
Strain ID
KOCMP-100102-Pcsk9-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pcsk9-KO Mouse (Catalog S-KO-00067) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pcsk9-KO
Strain ID
KOCMP-100102-Pcsk9-B6J-VA
Gene Name
Product ID
S-KO-00067
Gene Alias
FH3, PC9, Narc1, HCHOLA3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000049507
NCBI Transcript ID
NM_153565
Target Region
Exon 4~11
Size of Effective Region
~10.0 kb
Overview of Gene Research
Deleting the PCSK9 gene in mouse cancer cells substantially attenuates or prevents their growth in a manner that depends on cytotoxic T cells. It also enhances the efficacy of immune therapy targeted at the checkpoint protein PD1. Inhibiting PCSK9, either through genetic deletion or using PCSK9 antibodies, increases the expression of major histocompatibility protein class I (MHC I) proteins on the tumour cell surface, promoting robust intratumoral infiltration of cytotoxic T cells. Mechanistically, PCSK9 can disrupt the recycling of MHC I to the cell surface by associating with it physically and promoting its relocation and degradation in the lysosome [1].
In conclusion, PCSK9 is a key regulator of LDL-C levels through its impact on LDL receptor degradation. The gene knockout mouse models have revealed its unexpected role in enhancing cancer immune checkpoint therapy, suggesting PCSK9 as a potential target not only for hypercholesterolemia-related cardiovascular diseases but also for cancer treatment [1].
References:
1. Liu, Xinjian, Bao, Xuhui, Hu, Mengjie, Li, Fang, Li, Chuan-Yuan. 2020. Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer. In Nature, 588, 693-698. doi:10.1038/s41586-020-2911-7. https://pubmed.ncbi.nlm.nih.gov/33177715/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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