C57BL/6JCya-Slc35b3em1/Cya
Common Name
Slc35b3-KO
Product ID
S-KO-00602
Backgroud
C57BL/6JCya
Strain ID
KOCMP-108652-Slc35b3-B6J-VA
When using this mouse strain in a publication, please cite “Slc35b3-KO Mouse (Catalog S-KO-00602) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Slc35b3-KO
Strain ID
KOCMP-108652-Slc35b3-B6J-VA
Gene Name
Product ID
S-KO-00602
Gene Alias
CGI-19, PABST2, PAPST2, 4921526O06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 13
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021870
NCBI RefSeq
NM_001170431
Target Region
Exon 2~8
Size of Effective Region
~18.7 kb
Overview of Gene Research
Slc35b3, known as solute carrier family 35 member B3, is a transferase involved in the synthesis of 3'-phosphoadenosine-5'-phosphosulfate (PAPS), which plays a key role in mucin sulfation [1]. It is part of the aryl hydrocarbon receptor (AHR)-Papss2-Slc35b3 pathway, contributing to the maintenance of intestinal epithelial homeostasis [1].
In a study on inflammatory bowel disease (IBD), a high-fat diet (HFD) led to a decrease in indole-3-acetic acid (IAA) and intestinal barrier damage. IAA supplementation enhanced intestinal mucin sulfation and alleviated colitis by upregulating Papss2 and Slc35b3 via the AHR [1]. Oral administration of Lactobacillus reuteri, which produces IAA, protected against colitis and promoted mucin sulfation, while a modified strain lacking the ability to produce IAA did not [1]. In addition, in non-small cell lung cancer (NSCLC), there were nominally significant associations between copy number variations (CNVs) and the SLC35B3 gene [2]. Also, in muscle-invasive bladder cancer, a 9-gene signature including SLC35B3 could predict response to neoadjuvant chemotherapy [3].
In conclusion, Slc35b3 is crucial for maintaining intestinal epithelial homeostasis through its role in mucin sulfation. Its upregulation by IAA via the AHR pathway has implications for IBD treatment. Additionally, the associations of SLC35B3 with CNVs in NSCLC and its role in predicting chemotherapy response in muscle-invasive bladder cancer suggest its significance in cancer-related research.
References:
1. Li, Mengfan, Ding, Yiyun, Wei, Jingge, Su, Xiaomin, Cao, Hailong. 2024. Gut microbiota metabolite indole-3-acetic acid maintains intestinal epithelial homeostasis through mucin sulfation. In Gut microbes, 16, 2377576. doi:10.1080/19490976.2024.2377576. https://pubmed.ncbi.nlm.nih.gov/39068517/
2. Heo, Yeonjeong, Heo, Jeongwon, Han, Seon-Sook, Cheong, Hyun Sub, Hong, Yoonki. 2020. Difference of copy number variation in blood of patients with lung cancer. In The International journal of biological markers, 36, 3-9. doi:10.1177/1724600820980739. https://pubmed.ncbi.nlm.nih.gov/33307925/
3. Hepburn, Anastasia C, Lazzarini, Nicola, Veeratterapillay, Rajan, Bacardit, Jaume, Heer, Rakesh. 2021. Identification of CNGB1 as a Predictor of Response to Neoadjuvant Chemotherapy in Muscle-Invasive Bladder Cancer. In Cancers, 13, . doi:10.3390/cancers13153903. https://pubmed.ncbi.nlm.nih.gov/34359804/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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