C57BL/6JCya-Pp2d1em1/Cya
Common Name
Pp2d1-KO
Product ID
S-KO-00753
Backgroud
C57BL/6JCya
Strain ID
KOCMP-110332-Pp2d1-B6J-VA
When using this mouse strain in a publication, please cite “Pp2d1-KO Mouse (Catalog S-KO-00753) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pp2d1-KO
Strain ID
KOCMP-110332-Pp2d1-B6J-VA
Gene Name
Product ID
S-KO-00753
Gene Alias
4921523A10Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000056198
NCBI RefSeq
NM_173449
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Overview of Gene Research
Pp2d1 is a member of the metal-dependent protein phosphatase (PPM) family, also known as PP2C phosphatases. These phosphatases bind manganese/magnesium ions in their active center, function as single-subunit enzymes, and are involved in regulating various cellular functions such as cell cycle control, cell differentiation, immune responses, and cell metabolism [1].
In a study on large-scale discovery of male reproductive tract-specific genes, Pp2d1 was identified as a testis-specific gene. Using the CRISPR/Cas9 system to generate knockout mice, it was found that Pp2d1 is individually dispensable for male mouse fertility [2]. Another study on gene expression profiles in rats under extracorporeal membrane oxygenation showed that Pp2d1 had the highest expression of inflammation/immune suppression related genes in the spontaneous breathing group compared to mechanical ventilation groups, indicating its possible role in inflammation regulation in this context [3].
In summary, Pp2d1, as part of the PPM family, may play a role in multiple cellular processes. The knockout mouse model has helped to clarify its non-essential role in male mouse fertility. Its differential expression in different respiratory settings in rats also implies a potential function in inflammation/immune suppression, which provides insights into its biological functions and possible implications in related disease areas.
References:
1. Kamada, Rui, Kudoh, Fuki, Ito, Shogo, Omichinski, James G, Sakaguchi, Kazuyasu. 2020. Metal-dependent Ser/Thr protein phosphatase PPM family: Evolution, structures, diseases and inhibitors. In Pharmacology & therapeutics, 215, 107622. doi:10.1016/j.pharmthera.2020.107622. https://pubmed.ncbi.nlm.nih.gov/32650009/
2. Robertson, Matthew J, Kent, Katarzyna, Tharp, Nathan, Matzuk, Martin M, Garcia, Thomas X. 2020. Large-scale discovery of male reproductive tract-specific genes through analysis of RNA-seq datasets. In BMC biology, 18, 103. doi:10.1186/s12915-020-00826-z. https://pubmed.ncbi.nlm.nih.gov/32814578/
3. Mori, Shohei, Ohtsuka, Takashi, Hashimoto, Kohei, Shibazaki, Takamasa, Shimoda, Masayuki. 2025. Gene expression profiles in respiratory settings in rats under extracorporeal membrane oxygenation. In Journal of thoracic disease, 17, 31-41. doi:10.21037/jtd-24-1661. https://pubmed.ncbi.nlm.nih.gov/39975719/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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