C57BL/6JCya-Cebpeem1/Cya
Common Name:
Cebpe-KO
Product ID:
S-KO-00781
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cebpe-KO
Strain ID
KOCMP-110794-Cebpe-B6J-VA
Gene Name
Product ID
S-KO-00781
Gene Alias
C/EBPe; C/EBPepsilon; CRP1; Gm294
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cebpeem1/Cya mice (Catalog S-KO-00781) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064290
NCBI RefSeq
NM_207131
Target Region
Exon 1~2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Cebpe, also known as CCAAT enhancer-binding protein epsilon, is a crucial transcription factor involved in late myeloid lineage differentiation and cellular function [3]. It plays an essential role in granulocytic differentiation, regulating the controlled gene expression necessary for the maturation of granulocytes [4].
In functional studies, Cebpe-knockout mice exhibit defects in the terminal differentiation of granulocytes, similar to the phenotype of neutrophil-specific granule deficiency (SGD) in humans caused by Cebpe mutations [2,5]. In acute myeloid leukemia (AML), Cebpe expression is an independent prognostic factor for overall survival, event-free survival, and relapse. Low Cebpe expression is associated with a high relapse rate, and it can stratify wild-type patients of multiple genes into different outcome groups. Additionally, allogeneic transplantation has different effects on survival rates depending on Cebpe expression levels in AML patients [1].
In conclusion, Cebpe is vital for granulocytic differentiation. Studies using gene knockout mouse models have revealed its significance in normal hematopoietic cell development and in disease conditions such as SGD and AML. Understanding Cebpe's function provides insights into the mechanisms of myeloid cell differentiation and offers potential prognostic and therapeutic implications for related diseases.
References:
1. Li, Kening, Du, Yuxin, Wei, Dong-Qing, Zhang, Fang. 2019. CEBPE expression is an independent prognostic factor for acute myeloid leukemia. In Journal of translational medicine, 17, 188. doi:10.1186/s12967-019-1944-x. https://pubmed.ncbi.nlm.nih.gov/31164135/
2. Banday, Aaqib Zaffar, Kaur, Anit, Akagi, Tadayuki, Rawat, Amit, Singh, Surjit. 2022. A Novel CEBPE Variant Causes Severe Infections and Profound Neutropenia. In Journal of clinical immunology, 42, 1434-1450. doi:10.1007/s10875-022-01304-7. https://pubmed.ncbi.nlm.nih.gov/35726044/
3. Göös, Helka, Fogarty, Christopher L, Sahu, Biswajyoti, Taipale, Jussi, Seppänen, Mikko R J. 2019. Gain-of-function CEBPE mutation causes noncanonical autoinflammatory inflammasomopathy. In The Journal of allergy and clinical immunology, 144, 1364-1376. doi:10.1016/j.jaci.2019.06.003. https://pubmed.ncbi.nlm.nih.gov/31201888/
4. Shyamsunder, Pavithra, Sankar, Haresh, Mayakonda, Anand, Madan, Vikas, Koeffler, H Phillip. 2018. CARD10, a CEBPE target involved in granulocytic differentiation. In Haematologica, 103, 1269-1277. doi:10.3324/haematol.2018.190280. https://pubmed.ncbi.nlm.nih.gov/29773596/
5. Shyamsunder, Pavithra, Shanmugasundaram, Mahalakshmi, Mayakonda, Anand, Madan, Vikas, Koeffler, H Phillip. 2019. Identification of a novel enhancer of CEBPE essential for granulocytic differentiation. In Blood, 133, 2507-2517. doi:10.1182/blood.2018886077. https://pubmed.ncbi.nlm.nih.gov/30952671/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen