C57BL/6NCya-Chrnb2em1/Cya
Common Name:
Chrnb2-KO
Product ID:
S-KO-00858
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chrnb2-KO
Strain ID
KOCMP-11444-Chrnb2-B6N-VA
Gene Name
Product ID
S-KO-00858
Gene Alias
Acrb-2; Acrb2; C030030P04Rik; [b]2-nAchR
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Chrnb2em1/Cya mice (Catalog S-KO-00858) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029562
NCBI RefSeq
NM_009602
Target Region
Exon 2~5
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
CHRNB2, encoding the β2 subunit of the α4β2 nicotine acetylcholine receptor, is involved in regulating neurotransmitter release, thus playing a crucial role in various physiological and pathologic processes. It is associated with pathways such as the β-catenin, PI3K-AKT, and JAK-STAT pathways [1,2]. Genetic models, like gene knockout mouse models, can be used to study its functions.
In pancreatic cancer, CHRNB2 negatively relates to lymph node metastasis. Knockdown of CHRNB2 increases the migration and invasion of pancreatic cancer cells, while overexpression decreases these abilities, likely via inhibiting the β-catenin pathway [1]. In gastric cancer, knockdown of CHRNB2 attenuates cell proliferation, and knockout influences cell survival and metastasis-related functions. Therapeutic monoclonal antibodies targeting CHRNB2 can inhibit gastric cancer cell growth [2]. In adult male rats, nicotine vapor exposure reduces CHRNB2 expression, leading to increased risky choice [3].
In conclusion, CHRNB2 plays a significant role in regulating cell migration, invasion, and proliferation in cancer, as well as in behaviors related to nicotine exposure. Gene knockout-based research on CHRNB2 has provided insights into its functions in pancreatic and gastric cancers, and its association with nicotine-related behaviors, which could potentially contribute to the development of new therapeutic strategies for these diseases and nicotine-related disorders.
References:
1. Qin, Cheng, Li, Tianhao, Wang, Yuanyang, Zhao, Yutong, Wang, Weibin. 2022. CHRNB2 represses pancreatic cancer migration and invasion via inhibiting β-catenin pathway. In Cancer cell international, 22, 340. doi:10.1186/s12935-022-02768-8. https://pubmed.ncbi.nlm.nih.gov/36344976/
2. Kanda, Mitsuro, Shimizu, Dai, Nakamura, Shunsuke, Katsuno, Masahisa, Kodera, Yasuhiro. 2021. Blockade of CHRNB2 signaling with a therapeutic monoclonal antibody attenuates the aggressiveness of gastric cancer cells. In Oncogene, 40, 5495-5504. doi:10.1038/s41388-021-01945-9. https://pubmed.ncbi.nlm.nih.gov/34331011/
3. Giner, Priscilla, Maynez-Anchondo, Liliana, Liley, Anna E, Simon, Nicholas W, Mendez, Ian A. 2022. Increased Risky Choice and Reduced CHRNB2 Expression in Adult Male Rats Exposed to Nicotine Vapor. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031231. https://pubmed.ncbi.nlm.nih.gov/35163155/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen