C57BL/6NCya-Adh5em1/Cya
Common Name
Adh5-KO
Product ID
S-KO-00928
Backgroud
C57BL/6NCya
Strain ID
KOCMP-11532-Adh5-B6N-VA
When using this mouse strain in a publication, please cite “Adh5-KO Mouse (Catalog S-KO-00928) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Adh5-KO
Strain ID
KOCMP-11532-Adh5-B6N-VA
Gene Name
Product ID
S-KO-00928
Gene Alias
Adh-5, Adh3, GSNOR
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000005964
NCBI RefSeq
NM_007410
Target Region
Exon 5~6
Size of Effective Region
~0.6 kb
Overview of Gene Research
Adh5, also known as alcohol dehydrogenase 5 (class III), chi polypeptide, is the key enzyme metabolizing S-nitrosoglutathione (GSNO) to decrease cellular S-nitrosothiols (SNOs) [1]. It is involved in maintaining SNO homeostasis, which is crucial for innate immune responses, as it facilitates STING activation during oxidative burst [1]. It also plays a role in regulating the nitroso-redox status in brown adipose tissue (BAT), thus maintaining metabolic homeostasis [2].
In Adh5-deficient mice, there are defective STING-dependent immune responses upon microbial challenge, facilitating viral replication, indicating its importance in host defense [1]. In BAT, loss of Adh5 impairs cold-induced UCP1-dependent thermogenesis and worsens obesity-associated metabolic dysfunction [2]. In zebrafish, disruption of adh5 in fancd2 -/- zebrafish led to a body size reduction and a lower number of mature spermatozoa compared to wild-type or single knockout zebrafish [3].
In conclusion, Adh5 is essential for maintaining SNO homeostasis in innate immunity and nitroso-redox homeostasis in BAT metabolism. Gene knockout models in mice and zebrafish have revealed its critical roles in host defense against pathogens, adipose thermogenesis, and in the context of certain genetic disorders, providing insights into related disease mechanisms [1,2,3].
References:
1. Jia, Mutian, Chai, Li, Wang, Jie, Jia, Jihui, Zhao, Wei. 2024. S-nitrosothiol homeostasis maintained by ADH5 facilitates STING-dependent host defense against pathogens. In Nature communications, 15, 1750. doi:10.1038/s41467-024-46212-z. https://pubmed.ncbi.nlm.nih.gov/38409248/
2. Sebag, Sara C, Zhang, Zeyuan, Qian, Qingwen, Bartelt, Alexander, Yang, Ling. . ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue. In Cell reports, 37, 110003. doi:10.1016/j.celrep.2021.110003. https://pubmed.ncbi.nlm.nih.gov/34788615/
3. Mu, Anfeng, Cao, Zimu, Huang, Denggao, Maegawa, Shingo, Takata, Minoru. 2023. Effects of the major formaldehyde catalyzer ADH5 on phenotypes of fanconi anemia zebrafish model. In Molecular biology reports, 50, 8385-8395. doi:10.1007/s11033-023-08696-8. https://pubmed.ncbi.nlm.nih.gov/37615925/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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