C57BL/6JCya-Parp1em1/Cya
Common Name:
Parp1-KO
Product ID:
S-KO-00939
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Parp1-KO
Strain ID
KOCMP-11545-Parp1-B6J-VA
Gene Name
Product ID
S-KO-00939
Gene Alias
5830444G22Rik; ARTD1; Adprp; Adprt1; PARP; PPOL; msPARP; parp-1; sPARP-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Parp1em1/Cya mice (Catalog S-KO-00939) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027777
NCBI RefSeq
NM_007415
Target Region
Exon 2~4
Size of Effective Region
~6.4 kb
Detailed Document
Overview of Gene Research
Poly(ADP-ribose) polymerase 1 (PARP1), also known as ADPRT1, is a multifunctional human ADP-ribosyltransferase. It plays a key role in multiple DNA repair pathways such as base excision repair (BER), non-homologous end joining (NHEJ), homologous recombination (HR), and Okazaki-fragment processing pathways. In response to DNA strand breaks, PARP1 covalently attaches ADP-ribose moieties to itself and other proteins, recruiting DNA repair proteins to the damage site [2].
PARP1 UFMylation at K548 is required for efficient CHK1 activation during replication stress. Inactivation of UFL1, the E3 enzyme for UFMylation, leads to delayed CHK1 activation similar to PARP1-deficient cells. PARP1 UFMylation-deficient mutant (K548R) and pathogenic mutant (F553L) compromise CHK1 activation, replication fork restart, and chromosome stability. PARP1 UFMylation-deficient knock-in mice are more sensitive to replication stress from anticancer treatments, showing that PARP1 UFMylation promotes replication fork stability and safeguards genome integrity [1].
In conclusion, PARP1 is crucial for DNA repair and maintaining genomic integrity. Model-based research, especially using PARP1 UFMylation-deficient knock-in mice, reveals its role in replication stress response. Understanding PARP1 function has implications for treating cancers and other diseases related to DNA damage and replication stress.
References:
1. Gong, Yamin, Wang, Zhifeng, Zong, Wen, Wang, Zhao-Qi, Xu, Xingzhi. 2024. PARP1 UFMylation ensures the stability of stalled replication forks. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2322520121. doi:10.1073/pnas.2322520121. https://pubmed.ncbi.nlm.nih.gov/38657044/
2. Spiegel, Jacob O, Van Houten, Bennett, Durrant, Jacob D. 2021. PARP1: Structural insights and pharmacological targets for inhibition. In DNA repair, 103, 103125. doi:10.1016/j.dnarep.2021.103125. https://pubmed.ncbi.nlm.nih.gov/33940558/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen