C57BL/6JCya-Adra1bem1/Cya
Common Name:
Adra1b-KO
Product ID:
S-KO-00941
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Adra1b-KO
Strain ID
KOCMP-11548-Adra1b-B6J-VA
Gene Name
Product ID
S-KO-00941
Gene Alias
[a]1b
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adra1bem1/Cya mice (Catalog S-KO-00941) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000067258
NCBI RefSeq
NM_001284381
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Adra1b, the gene encoding the alpha-1b adrenergic receptor, is involved in the adrenergic signaling pathway. These receptors contribute to vasoconstriction in humans and are widely distributed in the peripheral and central nervous systems, playing important roles in regulating various physiological functions [3,5]. Genetic models, such as gene-knockout (KO) mouse models, are valuable tools for studying its function.
A KO mouse model with tissue-specific deletion of Adra1b in hepatocytes was generated to study its role in metabolism. Selective deletion of Adra1b in mouse liver had limited metabolic impact in lean mice. However, in female mice, loss of Adra1b in hepatocytes exacerbated diet-induced obesity, insulin resistance, and glucose intolerance, accompanied by reduced hepatic gluconeogenic capacity and adipose tissue reprogramming. This highlights the sex-dependent role of Adra1b in the sympathetic nervous system's regulation of energy and glucose homeostasis [2].
In conclusion, Adra1b plays a crucial role in vasoconstriction and is involved in the regulation of energy and glucose homeostasis, especially in female mice as revealed by the KO mouse model. Its study also has implications in understanding diseases like psoriasis, gastric cancer, and ADHD, where its gene variants or expression levels are associated with disease susceptibility or progression [1,4,6,7].
References:
1. Fan, Xing, Wang, Hongyan, Sun, Liangdan, Zhang, Xuejun, Schork, Nicholas J. 2019. Fine mapping and subphenotyping implicates ADRA1B gene variants in psoriasis susceptibility in a Chinese population. In Epigenomics, 11, 455-467. doi:10.2217/epi-2018-0131. https://pubmed.ncbi.nlm.nih.gov/30785334/
2. Silva, Anisia, Mouchiroud, Mathilde, Lavoie, Olivier, Elmquist, Joel K, Caron, Alexandre. 2024. Liver adrenoceptor alpha-1b plays a key role in energy and glucose homeostasis in female mice. In American journal of physiology. Endocrinology and metabolism, 327, E626-E635. doi:10.1152/ajpendo.00153.2024. https://pubmed.ncbi.nlm.nih.gov/39259165/
3. Adefurin, A, Ghimire, L V, Kohli, U, Stein, C M, Kurnik, D. 2016. Genetic variation in the alpha1B-adrenergic receptor and vascular response. In The pharmacogenomics journal, 17, 366-371. doi:10.1038/tpj.2016.29. https://pubmed.ncbi.nlm.nih.gov/27089938/
4. Noda, H, Miyaji, Y, Nakanishi, A, Konishi, F, Miki, Y. . Frequent reduced expression of alpha-1B-adrenergic receptor caused by aberrant promoter methylation in gastric cancers. In British journal of cancer, 96, 383-90. doi:. https://pubmed.ncbi.nlm.nih.gov/17242706/
5. Masukawa, Daiki, Takahagi, Ryo, Nakao, Yuka, Goshima, Yoshio. . L-DOPA Receptor GPR143 Functionally Couples with Adrenergic α1B Receptor at the Second Transmembrane Interface. In Biological & pharmaceutical bulletin, 46, 869-873. doi:10.1248/bpb.b23-00217. https://pubmed.ncbi.nlm.nih.gov/37394637/
6. Hawi, Ziarih, Matthews, Natasha, Barry, Edwina, Gill, Michael, Bellgrove, Mark A. 2012. A high density linkage disequilibrium mapping in 14 noradrenergic genes: evidence of association between SLC6A2, ADRA1B and ADHD. In Psychopharmacology, 225, 895-902. doi:10.1007/s00213-012-2875-x. https://pubmed.ncbi.nlm.nih.gov/23052569/
7. Wang, Tingan, Qin, Yuzhou, Lai, Hao, Huang, Mingwei, Chen, Jiansi. 2019. The prognostic value of ADRA1 subfamily genes in gastric carcinoma. In Oncology letters, 18, 3150-3158. doi:10.3892/ol.2019.10660. https://pubmed.ncbi.nlm.nih.gov/31452791/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen