C57BL/6JCya-Aicdaem1/Cya
Common Name
Aicda-KO
Product ID
S-KO-00969
Backgroud
C57BL/6JCya
Strain ID
KOCMP-11628-Aicda-B6J-VA
When using this mouse strain in a publication, please cite “Aicda-KO Mouse (Catalog S-KO-00969) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Aicda-KO
Strain ID
KOCMP-11628-Aicda-B6J-VA
Gene Name
Product ID
S-KO-00969
Gene Alias
Aid, Arp2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000043301
NCBI RefSeq
NM_009645
Target Region
Exon 1~5
Size of Effective Region
~8.6 kb
Overview of Gene Research
Aicda, encoding activation-induced cytidine deaminase (AID), is crucial for immunoglobulin (Ig) gene somatic hypermutation (SHM) and class switch DNA recombination (CSR). These processes are central to the maturation of effective antibody responses, occurring mainly in germinal centers. AID triggers DNA deamination, which underpins Ig variable gene conversion, isotype class switching, and some oncogenic translocations in B cell tumors [2,3].
In Aicda -deficient mice, IgA production is impaired. Feeding these mice a high-fat diet (HFD) exacerbates hyperinsulinemia, with increased gene expression of monocyte chemoattractant protein-1 in the liver, a chemokine related to hyperinsulinemia onset. However, Aicda deficiency does not lead to gut dysbiosis, though it does increase inflammatory cytokine gene expression in the ileum [1].
In conclusion, Aicda is essential for antibody-related processes like SHM and CSR. The use of Aicda-deficient mouse models has revealed its role in metabolic and inflammatory responses, specifically in the context of HFD-induced hyperinsulinemia and ileal inflammation, contributing to our understanding of related disease mechanisms.
References:
1. Tsuruta, Takeshi, Muhomah, Teresia Aluoch, Sonoyama, Kei, Katsumata, Emiko, Nishino, Naoki. 2021. Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice. In Nutrition research (New York, N.Y.), 93, 15-26. doi:10.1016/j.nutres.2021.06.009. https://pubmed.ncbi.nlm.nih.gov/34332207/
2. Di Noia, Javier M, Neuberger, Michael S. . Molecular mechanisms of antibody somatic hypermutation. In Annual review of biochemistry, 76, 1-22. doi:. https://pubmed.ncbi.nlm.nih.gov/17328676/
3. Xu, Zhenming, Pone, Egest J, Al-Qahtani, Ahmed, Zan, Hong, Casali, Paolo. . Regulation of aicda expression and AID activity: relevance to somatic hypermutation and class switch DNA recombination. In Critical reviews in immunology, 27, 367-97. doi:. https://pubmed.ncbi.nlm.nih.gov/18197815/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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