C57BL/6JCya-Atp9aem1/Cya
Common Name
Atp9a-KO
Product ID
S-KO-01175
Backgroud
C57BL/6JCya
Strain ID
KOCMP-11981-Atp9a-B6J-VA
When using this mouse strain in a publication, please cite “Atp9a-KO Mouse (Catalog S-KO-01175) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Atp9a-KO
Strain ID
KOCMP-11981-Atp9a-B6J-VA
Gene Name
Product ID
S-KO-01175
Gene Alias
IIa
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000109175
NCBI RefSeq
NM_001289445
Target Region
Exon 2~4
Size of Effective Region
~5.9 kb
Overview of Gene Research
ATP9A, a lipid flippase of the class II P4-ATPases, is involved in cellular vesicle trafficking, specifically in the recycling pathway from endosomes to the plasma membrane [2,3]. It plays a role in several biological processes and is associated with various disease conditions.
In hepatocellular carcinoma, nutrient starvation-induced macropinocytosis was found to be regulated by ATP9A. High ATP9A levels predicted poor patient outcomes. ATP9A interacted with ATP6V1A, facilitating its transport to the plasma membrane, promoting cholesterol accumulation, and driving RAC1-dependent macropinocytosis [1]. In addition, ATP9A deficiency in humans and Atp9a null mice led to neurodevelopmental disorders such as autosomal recessive hypotonia, intellectual disability, and attention deficit hyperactivity disorder (ADHD). Atp9a null mice recapitulated these symptoms, with abnormal neurite morphology and impaired synaptic transmission. ATP9A was shown to be required for maintaining neuronal neurite morphology and neural cell viability in vitro, and it regulated endosomal recycling by modulating RAB5 and RAB11 activation [2].
In summary, ATP9A is crucial for vesicle trafficking and endosomal recycling. Its dysfunction in gene-knockout mouse models is associated with hepatocellular carcinoma and neurodevelopmental disorders, highlighting its significance in understanding these disease mechanisms. [1,2]
References:
1. Wang, Xiaoqing, Li, Yue, Xiao, Yunyun, Lin, Ye, Guan, Jian. 2023. The phospholipid flippase ATP9A enhances macropinocytosis to promote nutrient starvation tolerance in hepatocellular carcinoma. In The Journal of pathology, 260, 17-31. doi:10.1002/path.6059. https://pubmed.ncbi.nlm.nih.gov/36715683/
2. Meng, Tian, Chen, Xiaoting, He, Zhengjie, Yan, Yousheng, Feng, Du. 2023. ATP9A deficiency causes ADHD and aberrant endosomal recycling via modulating RAB5 and RAB11 activity. In Molecular psychiatry, 28, 1219-1231. doi:10.1038/s41380-022-01940-w. https://pubmed.ncbi.nlm.nih.gov/36604604/
3. Tanaka, Yoshiki, Ono, Natsuki, Shima, Takahiro, Takatsu, Hiroyuki, Shin, Hye-Won. 2016. The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane. In Molecular biology of the cell, 27, 3883-3893. doi:. https://pubmed.ncbi.nlm.nih.gov/27733620/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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