C57BL/6JCya-Bak1em1/Cya
Common Name:
Bak1-KO
Product ID:
S-KO-01196
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bak1-KO
Strain ID
KOCMP-12018-Bak1-B6J-VA
Gene Name
Product ID
S-KO-01196
Gene Alias
Bak; N-BAK1; N-Bak
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bak1em1/Cya mice (Catalog S-KO-01196) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000078691
NCBI RefSeq
NM_007523
Target Region
Exon 3~4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
BAK1, also known as BCL2 homologous antagonist/killer 1, is a key pro-apoptotic effector protein encoded by the human BAK1 gene. It is a critical regulator in the apoptotic machinery, with its protein structure and function closely related to apoptosis. BAK1 is involved in mitochondrial outer membrane permeabilization (MOMP), a central step in apoptosis execution, and its function is intertwined with other members of the BCL2 family, such as BAX [3,5].
Studies have revealed several associations related to BAK1. The rs210138 polymorphism in BAK1 is associated with an increased risk of testicular germ cell tumors (TGCT), where the G allele compared to A increases the risk [1]. In preeclampsia, hsa_circ_0002348 regulates trophoblast proliferation and apoptosis through the miR-126-3p/BAK1 axis. Hsa_circ_0002348 acts as a sponge for miR-126-3p, upregulating BAK1 expression, which in turn affects trophoblast function [2]. Regarding abdominal aortic aneurysms (AAA), specific BAK1 single-nucleotide polymorphisms (SNPs) were found in aneurysmic and healthy aortic tissues but not in matching blood samples, suggesting a role of gene selection in AAA ontogeny [4]. A 4-bp deletion polymorphism in an enhancer adjacent to BAK1 (rs3831846) reduces enhancer activity and confers risk of intracranial germ cell tumors (IGCTs), and it is in strong linkage disequilibrium with a TGCTs susceptibility variant [6].
In conclusion, BAK1 plays a crucial role in apoptosis, and its genetic variations are associated with various diseases including testicular and intracranial germ cell tumors, preeclampsia, and abdominal aortic aneurysms. These disease-related associations highlight the importance of understanding BAK1's function and genetic polymorphisms, which may provide insights into disease mechanisms and potential therapeutic targets.
References:
1. Qin, Jiaxuan, Yang, Yufeng, Zhuang, Xuan, Xing, Jinchun. 2020. Association Between BAK1 Gene rs210138 Polymorphisms and Testicular Germ Cell Tumors: A Systematic Review and Meta-Analysis. In Frontiers in endocrinology, 11, 2. doi:10.3389/fendo.2020.00002. https://pubmed.ncbi.nlm.nih.gov/32038496/
2. Zhou, Jizi, Zhao, Ying, An, Ping, Li, Xiaotian, Xiong, Yu. 2023. Hsa_circ_0002348 regulates trophoblast proliferation and apoptosis through miR-126-3p/BAK1 axis in preeclampsia. In Journal of translational medicine, 21, 509. doi:10.1186/s12967-023-04240-1. https://pubmed.ncbi.nlm.nih.gov/37507742/
3. Cosentino, Katia, Hertlein, Vanessa, Jenner, Andreas, Ries, Jonas, Garcia-Saez, Ana J. 2022. The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation. In Molecular cell, 82, 933-949.e9. doi:10.1016/j.molcel.2022.01.008. https://pubmed.ncbi.nlm.nih.gov/35120587/
4. Gottlieb, Bruce, Chalifour, Lorraine E, Mitmaker, Benjamin, Bkaily, Ghassan, Schweitzer, Morris. . BAK1 gene variation and abdominal aortic aneurysms. In Human mutation, 30, 1043-7. doi:10.1002/humu.21046. https://pubmed.ncbi.nlm.nih.gov/19514060/
5. Dadsena, Shashank, Cuevas Arenas, Rodrigo, Vieira, Gonçalo, Melo, Manuel N, García-Sáez, Ana J. 2024. Lipid unsaturation promotes BAX and BAK pore activity during apoptosis. In Nature communications, 15, 4700. doi:10.1038/s41467-024-49067-6. https://pubmed.ncbi.nlm.nih.gov/38830851/
6. Sonehara, Kyuto, Kimura, Yui, Nakano, Yoshiko, Okada, Yukinori, Terashima, Keita. 2022. A common deletion at BAK1 reduces enhancer activity and confers risk of intracranial germ cell tumors. In Nature communications, 13, 4478. doi:10.1038/s41467-022-32005-9. https://pubmed.ncbi.nlm.nih.gov/35918310/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen