C57BL/6JCya-Bmxem1/Cya
Common Name:
Bmx-KO
Product ID:
S-KO-01240
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Bmx-KO
Strain ID
KOCMP-12169-Bmx-B6J-VA
Gene Name
Product ID
S-KO-01240
Gene Alias
Etk; Etk/Bmx; Tyro8
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bmxem1/Cya mice (Catalog S-KO-01240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112265
NCBI RefSeq
NM_009759
Target Region
Exon 2~4
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Bmx, also known as Bone marrow kinase on chromosome X, is a cytosolic tyrosine kinase and a member of the TEC kinase family. It plays diverse roles in biological processes. In the immune response, Bmx is expressed in hematopoietic cells of the myeloid lineage. It is also involved in the response to ischemia and pressure overload in the endocardium and cardiac endothelium. In cancer, Bmx has been shown to mediate the survival and tumorigenicity of glioblastoma cancer stem cells. Bmx regulates the secretion of proinflammatory cytokines induced by TNFα, IL-1β, and TLR agonists in the inflammatory response, and is required for the activation of the MAP kinase and NFκB pathways [1].
In cecal ligation and puncture models of sepsis, Bmx-KO mice showed increased lung injury, inflammation, and thrombin-mediated endothelial permeability. Bmx specifically repressed thrombin-PAR1 signaling in endothelial cells by directly phosphorylating PAR1 and promoting its internalization and deactivation, thus protecting against vascular leakage during early sepsis [2]. In a mouse model of arthritis, Bmx deficiency conferred protection, and genetic replacement with a kinase-inactive allele restored susceptibility, suggesting that in vivo Bmx kinase activity can be dispensable [1]. In retinal ischemia/reperfusion injury experiments using Bmx-/-mice, Bmx knockdown mitigated negative impacts on retinal tissue structure and visual function, reduced apoptosis, and suppressed inflammatory responses through down-regulating phosphorylation of the AKT/ERK/STAT3 pathway [3].
In conclusion, Bmx is crucial in inflammation, cardiovascular disease, and cancer. Gene knockout mouse models have revealed its roles in these areas, such as its function in maintaining endothelial integrity during sepsis, its dispensable kinase activity in arthritis, and its impact on retinal injury. These findings contribute to understanding the biological functions of Bmx and may provide potential targets for related disease treatments.
References:
1. Cenni, Bruno, Gutmann, Sascha, Gottar-Guillier, Marie. . BMX and its role in inflammation, cardiovascular disease, and cancer. In International reviews of immunology, 31, 166-73. doi:10.3109/08830185.2012.663838. https://pubmed.ncbi.nlm.nih.gov/22449076/
2. Li, Zhao, Yin, Mingzhu, Zhang, Haifeng, Zhou, Huanjiao Jenny, Min, Wang. 2020. BMX Represses Thrombin-PAR1-Mediated Endothelial Permeability and Vascular Leakage During Early Sepsis. In Circulation research, 126, 471-485. doi:10.1161/CIRCRESAHA.119.315769. https://pubmed.ncbi.nlm.nih.gov/31910739/
3. Huang, Guangyi, Zhang, Shaoyang, Liao, Jing, Wei, Yantao, Xu, Fan. 2024. BMX deletion mitigates neuroinflammation induced by retinal ischemia/reperfusion through modulation of the AKT/ERK/STAT3 signaling cascade. In Heliyon, 10, e27114. doi:10.1016/j.heliyon.2024.e27114. https://pubmed.ncbi.nlm.nih.gov/38434304/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen