C57BL/6JCya-Runx2em1/Cya
Common Name
Runx2-KO
Product ID
S-KO-01353
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12393-Runx2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Runx2-KO Mouse (Catalog S-KO-01353) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Runx2-KO
Strain ID
KOCMP-12393-Runx2-B6J-VA
Gene Name
Product ID
S-KO-01353
Gene Alias
Cbf, LS3, AML3, Osf2, Cbfa1, Cbfa-1, PEBP2aA, Pebp2a1, Pebpa2a, CBF-alpha-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000160673
NCBI RefSeq
NM_009820.5
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Overview of Gene Research
Runx2, also known as runt-related transcription factor 2, is a crucial transcription factor for skeletal development. It is expressed in multipotent mesenchymal cells, osteoblast-lineage cells, and chondrocytes. Runx2 plays a major role in chondrocyte maturation, osteoblast differentiation, and is required for the proliferation of osteoprogenitors. It is involved in multiple signaling pathways such as hedgehog, Fgf, Wnt, and Pthlh, which are essential for bone development [2,3]. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
In VC associated with CKD, SIRT6-transgenic (SIRT6-Tg) mice showed alleviated VC, while vascular smooth muscle cell-specific (VSMC-specific) SIRT6 knocked-down mice showed severe VC. SIRT6 suppressed the osteogenic transdifferentiation of VSMCs via regulation of Runx2, demonstrating that Runx2 is involved in the osteogenic transdifferentiation process in this disease context [1].
In conclusion, Runx2 is essential for chondrocyte and osteoblast-lineage cell proliferation and differentiation, playing a key role in skeletal development. Mouse models, such as those related to SIRT6-Runx2 interactions in CKD-associated VC, have provided insights into the role of Runx2 in disease conditions, highlighting its importance in understanding and potentially treating related diseases.
References:
1. Li, Wenxin, Feng, Weijing, Su, Xiaoyan, Liu, Baohua, Huang, Hui. . SIRT6 protects vascular smooth muscle cells from osteogenic transdifferentiation via Runx2 in chronic kidney disease. In The Journal of clinical investigation, 132, . doi:10.1172/JCI150051. https://pubmed.ncbi.nlm.nih.gov/34793336/
2. Komori, Toshihisa. . Molecular Mechanism of Runx2-Dependent Bone Development. In Molecules and cells, 43, 168-175. doi:10.14348/molcells.2019.0244. https://pubmed.ncbi.nlm.nih.gov/31896233/
3. Komori, Toshihisa. 2024. Regulation of Skeletal Development and Maintenance by Runx2 and Sp7. In International journal of molecular sciences, 25, . doi:10.3390/ijms251810102. https://pubmed.ncbi.nlm.nih.gov/39337587/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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