C57BL/6JCya-Cblem1/Cya
Common Name:
Cbl-KO
Product ID:
S-KO-01358
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cbl-KO
Strain ID
KOCMP-12402-Cbl-B6J-VA
Gene Name
Product ID
S-KO-01358
Gene Alias
4732447J05Rik; Cbl-2; c-Cbl; cbl
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cblem1/Cya mice (Catalog S-KO-01358) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000206720
NCBI RefSeq
NM_007619
Target Region
Exon 4
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
Cbl, short for Casitas B lineage lymphoma, is a multifunctional protein with E3 ubiquitin ligase activity. It can degrade diverse proteins and plays a key role in different cell pathways, mainly related to cancer onset and progression, hematopoietic development, T cell receptor regulation, and muscle atrophy [2,3,5]. In plants, the CBL-CIPK network, where CBL is a calcineurin B-like protein, mediates Ca²⁺-related signaling pathways in response to abiotic and biotic stimuli [6].
In muscle atrophy, Cbl-b ubiquitinates and induces the degradation of IRS-1, a key intermediate in the IGF-1 signaling, which is crucial in unloading muscle atrophy in spaceflight and bedridden patients [1]. In cancer, somatic CBL mutations have been reported in various malignancies, and germline CBL mutations configure the so-called CBL syndrome, a cancer-predisposing condition [2]. Also, c-Cbl is considered a critical regulator and potential target in angiogenesis and human solid organ tumors [3]. The E3 ubiquitin ligase Cbl-b is a novel target in immune-oncology, modulating immune responses by facilitating ubiquitination and degradation of key signaling proteins [4].
In conclusion, Cbl is essential in multiple biological processes. Its role in muscle atrophy, cancer, angiogenesis, and immune-oncology has been revealed through various studies. Understanding Cbl function through gene-based research models can potentially provide new therapeutic strategies for related diseases.
References:
1. Nikawa, Takeshi, Ishidoh, Kazumi. 2020. Ubiquitin ligase Cbl-b and inhibitory Cblin peptides. In Biochimica et biophysica acta. Proteins and proteomics, 1868, 140495. doi:10.1016/j.bbapap.2020.140495. https://pubmed.ncbi.nlm.nih.gov/32663526/
2. Leardini, Davide, Messelodi, Daria, Muratore, Edoardo, Pession, Andrea, Masetti, Riccardo. 2022. Role of CBL Mutations in Cancer and Non-Malignant Phenotype. In Cancers, 14, . doi:10.3390/cancers14030839. https://pubmed.ncbi.nlm.nih.gov/35159106/
3. Lyle, Chimera L, Belghasem, Mostafa, Chitalia, Vipul C. 2019. c-Cbl: An Important Regulator and a Target in Angiogenesis and Tumorigenesis. In Cells, 8, . doi:10.3390/cells8050498. https://pubmed.ncbi.nlm.nih.gov/31126146/
4. Fusco, Riccardo, Saedi, Zeinab, Capriello, Imma, Lubskyy, Andriy, Dömling, Alexander. 2024. CBL-B - An upcoming immune-oncology target. In Expert opinion on therapeutic patents, 35, 47-64. doi:10.1080/13543776.2024.2412567. https://pubmed.ncbi.nlm.nih.gov/39582379/
5. Ren, Jiaqi, Lv, Linlin, Tao, Xufeng, Dong, Deshi, Liu, Jing. 2024. The role of CBL family ubiquitin ligases in cancer progression and therapeutic strategies. In Frontiers in pharmacology, 15, 1432545. doi:10.3389/fphar.2024.1432545. https://pubmed.ncbi.nlm.nih.gov/39130630/
6. Yu, Qinyang, An, Lijia, Li, Wenli. 2013. The CBL-CIPK network mediates different signaling pathways in plants. In Plant cell reports, 33, 203-14. doi:10.1007/s00299-013-1507-1. https://pubmed.ncbi.nlm.nih.gov/24097244/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen