C57BL/6JCya-Cd34em1/Cya
Common Name
Cd34-KO
Product ID
S-KO-01403
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12490-Cd34-B6J-VA
Status
When using this mouse strain in a publication, please cite “Cd34-KO Mouse (Catalog S-KO-01403) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Cd34-KO
Strain ID
KOCMP-12490-Cd34-B6J-VA
Gene Name
Product ID
S-KO-01403
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000016638
NCBI RefSeq
NM_133654
Target Region
Exon 2~3
Size of Effective Region
~3.1 kb
Overview of Gene Research
CD34 is a cell surface antigen expressed in numerous stem/progenitor cells, such as hematopoietic stem cells (HSCs) and endothelial progenitor cells (EPCs) [1]. It has been implicated in various biological processes, including angiogenesis, anti-inflammatory responses, immunomodulation, and anti-apoptosis/fibrosis roles [1]. Genetic models, like mouse models, are valuable for studying its functions.
In murine models, studies using Cd34-CreERT2; Rosa26-tdTomato and Cd34-CreERT2; Rosa26-eGFP-DTA mice have shown that non-bone-marrow-derived CD34+ cells give rise to fibroblasts and endothelial cells, while bone-marrow-derived CD34+ cells turn into immune cells in response to pressure overload. Partial depletion of CD34+ cells alleviated myocardial fibrosis and improved cardiac function in the Cd34-CreERT2; Rosa26-eGFP-DTA model, indicating CD34+ cells' role in cardiac fibrosis during heart failure [2]. Another study with a Cd34-CreERT2; R26-DTA mouse model demonstrated that depletion of Cd34+ cells could alleviate ventricular fibrosis after ischemia/reperfusion (I/R) injury and improve cardiac function, suggesting CD34+ cells contribute to cardiac remodeling after I/R injury [3].
In conclusion, CD34+ cells play significant roles in processes like cardiac repair, angiogenesis, and fibrosis. Model-based research, especially using Cd34 KO/CKO mouse models, has revealed their importance in heart-related diseases such as heart failure and cardiac remodeling after I/R injury, providing insights into potential therapeutic strategies for these conditions [2,3].
References:
1. Hassanpour, Mehdi, Salybekov, Amankeldi A, Kobayashi, Shuzo, Asahara, Takayuki. 2023. CD34 positive cells as endothelial progenitor cells in biology and medicine. In Frontiers in cell and developmental biology, 11, 1128134. doi:10.3389/fcell.2023.1128134. https://pubmed.ncbi.nlm.nih.gov/37138792/
2. Du, Luping, Sun, Xiaotong, Gong, Hui, Chen, Ting, Xu, Qingbo. 2023. Single cell and lineage tracing studies reveal the impact of CD34+ cells on myocardial fibrosis during heart failure. In Stem cell research & therapy, 14, 33. doi:10.1186/s13287-023-03256-0. https://pubmed.ncbi.nlm.nih.gov/36805782/
3. Xie, Jun, Jiang, Liujun, Wang, Junzhuo, Xu, Biao, Xu, Qingbo. 2023. Multilineage contribution of CD34+ cells in cardiac remodeling after ischemia/reperfusion injury. In Basic research in cardiology, 118, 17. doi:10.1007/s00395-023-00981-8. https://pubmed.ncbi.nlm.nih.gov/37147443/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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