C57BL/6JCya-Cd69em1/Cya
Common Name
Cd69-KO
Product ID
S-KO-01428
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12515-Cd69-B6J-VB
When using this mouse strain in a publication, please cite “Cd69-KO Mouse (Catalog S-KO-01428) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cd69-KO
Strain ID
KOCMP-12515-Cd69-B6J-VB
Gene Name
Product ID
S-KO-01428
Gene Alias
5830438K24Rik, AIM, VEA
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000032259
NCBI RefSeq
NM_001033122.4
Target Region
Exon 2~4
Size of Effective Region
~2.6 kb
Overview of Gene Research
Cd69, also known as activation inducer molecule, very early activation antigen, MLR-3 and Leu-23, is a membrane-bound, type II C-lectin receptor. It is a classical early marker of lymphocyte activation, rapidly appearing on the plasma membrane surface after stimulation. Cd69 is expressed by multiple tissue-resident immune cell subsets and is thus considered a marker of tissue retention. It regulates T-cell subset functions, determining migration-retention ratios and the acquisition of effector or regulatory phenotypes. It is involved in pathways related to Treg cell differentiation and cytokine secretion such as IFN-γ, IL-17, and IL-22 [1].
In Cd69 -/- mice, after myocardial infarction, there was a strong IL-17+ γδT cell response, increasing myocardial inflammation and worsening cardiac function, while CD69+ Tregs induced apoptosis and decreased IL-17A production in γδT cells [2]. In colitis mouse models, iTregs from CD4 Cre CD69 fl/fl mice failed to alleviate colitis, indicating that Cd69 is crucial for the immunosuppressive function of Tregs [3].
In conclusion, Cd69 is a key regulator in the immune system, influencing T-cell activation, differentiation, and retention. Gene knockout mouse models have revealed its importance in diseases like myocardial infarction and colitis, providing insights into its role in immune-related pathological processes and suggesting its potential as a therapeutic target.
References:
1. Cibrián, Danay, Sánchez-Madrid, Francisco. . CD69: from activation marker to metabolic gatekeeper. In European journal of immunology, 47, 946-953. doi:10.1002/eji.201646837. https://pubmed.ncbi.nlm.nih.gov/28475283/
2. Blanco-Domínguez, Rafael, de la Fuente, Hortensia, Rodríguez, Cristina, Martínez-González, José, Martín, Pilar. 2022. CD69 expression on regulatory T cells protects from immune damage after myocardial infarction. In The Journal of clinical investigation, 132, . doi:10.1172/JCI152418. https://pubmed.ncbi.nlm.nih.gov/36066993/
3. Yu, Lei, Zhou, Bingluo, Zhu, Yiran, Wang, Jianli, Jin, Hongchuan. 2023. HSF1 promotes CD69+ Treg differentiation to inhibit colitis progression. In Theranostics, 13, 1892-1905. doi:10.7150/thno.78078. https://pubmed.ncbi.nlm.nih.gov/37064870/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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