C57BL/6JCya-Cluem1/Cya
Common Name
Clu-KO
Product ID
S-KO-01533
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12759-Clu-B6J-VA
Status
When using this mouse strain in a publication, please cite “Clu-KO Mouse (Catalog S-KO-01533) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Clu-KO
Strain ID
KOCMP-12759-Clu-B6J-VA
Gene Name
Product ID
S-KO-01533
Gene Alias
Cli, ApoJ, Sgp2, SP-40, Sgp-2, Sugp-2, D14Ucla3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 14 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000022616
NCBI Transcript ID
NM_013492
Target Region
Exon 4~5
Size of Effective Region
~1.9 kb
Overview of Gene Research
CLU, also known as clusterin and apolipoprotein J (apoJ), is a highly evolutionary conserved glycoprotein. It functions as a molecular chaperone, involved in a broad range of physiological and pathophysiological processes, exerting a cytoprotective role [2]. It participates in regulating programmed cell death, metastasis, invasion, proliferation, and cell growth via diverse signaling pathways in cancer [3]. In the context of Alzheimer's disease, it is related to processes like amyloid-beta handling and neuroinflammation [4].
In oral cancer cells, CLU localizes to mitochondria and acts as an adaptor protein to induce mitophagy, controlling mitochondrial damage. It coordinates with BAX and LC3 to recruit autophagic machinery around damaged mitochondria in response to cisplatin treatment [1]. In oral cancer stem cells, cisplatin treatment activates higher mitophagy through regulating CLU levels. CLU promotes mitophagic degradation of MSX2, maintaining SOX2-mediated stemness [5].
In conclusion, CLU plays essential roles in cancer cell survival by regulating mitophagy and mitochondrial biogenesis in oral cancer [1]. It also has a significant impact on the stemness of oral cancer stem cells through mitophagic regulation [5]. These findings from in-vitro studies related to cancer cells contribute to understanding the role of CLU in cancer, potentially guiding the development of targeted cancer therapies.
References:
1. Praharaj, Prakash P, Patra, Srimanta, Singh, Amruta, Chae, Han J, Bhutia, Sujit K. 2024. CLU (clusterin) and PPARGC1A/PGC1α coordinately control mitophagy and mitochondrial biogenesis for oral cancer cell survival. In Autophagy, 20, 1359-1382. doi:10.1080/15548627.2024.2309904. https://pubmed.ncbi.nlm.nih.gov/38447939/
2. Rohne, Philipp, Prochnow, Hans, Koch-Brandt, Claudia. . The CLU-files: disentanglement of a mystery. In Biomolecular concepts, 7, 1-15. doi:10.1515/bmc-2015-0026. https://pubmed.ncbi.nlm.nih.gov/26673020/
3. Zhang, Yefei, Lv, Xiang, Chen, Liming, Liu, Yan. 2022. The role and function of CLU in cancer biology and therapy. In Clinical and experimental medicine, 23, 1375-1391. doi:10.1007/s10238-022-00885-2. https://pubmed.ncbi.nlm.nih.gov/36098834/
4. Uddin, Md Sahab, Kabir, Md Tanvir, Begum, Mst Marium, Behl, Tapan, Ashraf, Ghulam Md. 2020. Exploring the Role of CLU in the Pathogenesis of Alzheimer's Disease. In Neurotoxicity research, 39, 2108-2119. doi:10.1007/s12640-020-00271-4. https://pubmed.ncbi.nlm.nih.gov/32820456/
5. Praharaj, Prakash P, Patra, Srimanta, Mishra, Soumya R, Patil, Shankargouda, Bhutia, Sujit K. 2023. CLU (clusterin) promotes mitophagic degradation of MSX2 through an AKT-DNM1L/Drp1 axis to maintain SOX2-mediated stemness in oral cancer stem cells. In Autophagy, 19, 2196-2216. doi:10.1080/15548627.2023.2178876. https://pubmed.ncbi.nlm.nih.gov/36779631/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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