C57BL/6NCya-Cyp1b1em1/Cya
Common Name:
Cyp1b1-KO
Product ID:
S-KO-01707
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Cyp1b1-KO
Strain ID
KOCMP-13078-Cyp1b1-B6N-VA
Gene Name
Product ID
S-KO-01707
Gene Alias
CP1B; CYPIB1; P4501b1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cyp1b1em1/Cya mice (Catalog S-KO-01707) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024894
NCBI RefSeq
NM_009994
Target Region
Exon 2~3
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
Cyp1b1, a member of the cytochrome P450 superfamily, is a heme-thiolate monooxygenase. It is involved in the metabolism of various substances such as estradiol, retinol, and procarcinogens [3,4]. By catalyzing the hydroxylation of 17β-estradiol, it generates carcinogenic metabolites, playing a role in carcinogenesis and cancer progression [3]. It is also associated with the arachidonic acid metabolism pathway [1].
In multiple disease contexts, research has provided insights into Cyp1b1's functions. In colorectal cancer, CYP1B1-derived 20-HETE activates the protein kinase C pathway, increasing FBXO10 expression which promotes ACSL4 ubiquitination and degradation, leading to ferroptosis resistance and anti-PD-1 resistance. Inhibiting CYP1B1 sensitized tumor cells to anti-PD-1 antibody in a mouse model [1]. In liver fibrosis, systemic or HSC-specific ablation and pharmacological inhibition of CYP1B1 attenuated HSC activation and protected male mice from liver fibrosis induced by various agents. This was due to an increase in trehalose in CYP1B1-deficient HSCs resulting from intestinal suppression of trehalase [2]. In ovarian cancer, down-regulating CYP1B1 expression or using specific inhibitors decreased the cellular response to Olaparib, suggesting CYP1B1 promotes PARPi-resistance through histone H1.4 interaction and increased chromatin accessibility [5].
In conclusion, Cyp1b1 is crucial in processes related to cancer and fibrosis. Gene knockout or inhibition models in mice have revealed its role in ferroptosis regulation in cancer, as well as in liver fibrosis development, providing potential therapeutic targets for colorectal cancer, liver fibrosis, and ovarian cancer treatment.
References:
1. Chen, Congcong, Yang, Yabing, Guo, Yanguan, Pan, Jinghua, Pan, Yunlong. 2023. CYP1B1 inhibits ferroptosis and induces anti-PD-1 resistance by degrading ACSL4 in colorectal cancer. In Cell death & disease, 14, 271. doi:10.1038/s41419-023-05803-2. https://pubmed.ncbi.nlm.nih.gov/37059712/
2. Tung, Hung-Chun, Kim, Jong-Won, Zhu, Junjie, Patterson, Andrew D, Xie, Wen. 2024. Inhibition of heme-thiolate monooxygenase CYP1B1 prevents hepatic stellate cell activation and liver fibrosis by accumulating trehalose. In Science translational medicine, 16, eadk8446. doi:10.1126/scitranslmed.adk8446. https://pubmed.ncbi.nlm.nih.gov/39321267/
3. Fabris, Marciéli, Luiza Silva, Mariana, de Santiago-Silva, Kaio Maciel, de Lima Ferreira Bispo, Marcelle, Goes Camargo, Priscila. . CYP1B1: A Promising Target in Cancer Drug Discovery. In Anti-cancer agents in medicinal chemistry, 23, 981-988. doi:10.2174/1871520623666230119103914. https://pubmed.ncbi.nlm.nih.gov/36655529/
4. Faiq, Muneeb A, Dada, Rima, Sharma, Reetika, Saluja, Daman, Dada, Tanuj. . CYP1B1: a unique gene with unique characteristics. In Current drug metabolism, 15, 893-914. doi:. https://pubmed.ncbi.nlm.nih.gov/25658124/
5. Xue, Yite, Yin, Taotao, Yuan, Shuo, Cheng, Xiaodong, Wang, Hui. 2024. CYP1B1 promotes PARPi-resistance via histone H1.4 interaction and increased chromatin accessibility in ovarian cancer. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 77, 101151. doi:10.1016/j.drup.2024.101151. https://pubmed.ncbi.nlm.nih.gov/39395328/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen