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C57BL/6JCya-Dnase2aem1/Cya
Common Name:
Dnase2a-KO
Product ID:
S-KO-01782
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Dnase2a-KO
Strain ID
KOCMP-13423-Dnase2a-B6J-VA
Gene Name
Dnase2a
Product ID
S-KO-01782
Gene Alias
Dnase2
Background
C57BL/6JCya
NCBI ID
13423
Modification
Conventional knockout
Chromosome
8
Phenotype
MGI:1329019
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dnase2aem1/Cya mice (Catalog S-KO-01782) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003910
NCBI RefSeq
NM_010062
Target Region
Exon 1~5
Size of Effective Region
~1.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Dnase2a, also known as DNase II-alpha, is a lysosomal nuclease. Its primary function is to degrade DNA. It is involved in pathways such as the autophagosome-lysosomal pathway for DNA clearance and is crucial for several biological processes. Genetic models like Dnase2a knockout (KO) mice are valuable for studying its functions [2,3,4,5,6].

In Dnase2a KO mice, undegraded DNA accumulates in both phagocytic and nonphagocytic cells. In nonphagocytic cells, the excess DNA is from damaged nuclear DNA, and its removal requires nuclear export and autophagy-mediated delivery to lysosomes. This accumulated DNA can induce inflammation via the Sting cytosolic DNA-sensing pathway [2]. In atherosclerosis regression, silencing macrophage Dnase2a blocks efferocyte proliferation, apoptotic cell clearance, and plaque stabilization [1]. Dnase2a KO mice also suffer from severe anemia, as it is critical in definitive erythropoiesis where it digests the DNA of extruded nuclei of red blood cells during maturation [4].

In conclusion, Dnase2a is essential for DNA degradation in multiple cellular processes. Studies using Dnase2a KO mouse models have revealed its significance in conditions like inflammation, atherosclerosis, and anemia, highlighting its potential as a target for understanding and treating related diseases.

References:

1. Gerlach, Brennan D, Ampomah, Patrick B, Yurdagul, Arif, Tao, Wei, Tabas, Ira. 2021. Efferocytosis induces macrophage proliferation to help resolve tissue injury. In Cell metabolism, 33, 2445-2463.e8. doi:10.1016/j.cmet.2021.10.015. https://pubmed.ncbi.nlm.nih.gov/34784501/

2. Lan, Yuk Yuen, Londoño, Diana, Bouley, Richard, Rooney, Michael S, Hacohen, Nir. 2014. Dnase2a deficiency uncovers lysosomal clearance of damaged nuclear DNA via autophagy. In Cell reports, 9, 180-192. doi:10.1016/j.celrep.2014.08.074. https://pubmed.ncbi.nlm.nih.gov/25284779/

3. Lan, Yuk Yuen, Heather, James M, Eisenhaure, Thomas, Raychowdhury, Raktima, Hacohen, Nir. 2019. Extranuclear DNA accumulates in aged cells and contributes to senescence and inflammation. In Aging cell, 18, e12901. doi:10.1111/acel.12901. https://pubmed.ncbi.nlm.nih.gov/30706626/

4. Porcu, Susanna, Manchinu, Maria F, Marongiu, Maria F, Grosveld, Frank, Ristaldi, Maria S. 2011. Klf1 affects DNase II-alpha expression in the central macrophage of a fetal liver erythroblastic island: a non-cell-autonomous role in definitive erythropoiesis. In Molecular and cellular biology, 31, 4144-54. doi:10.1128/MCB.05532-11. https://pubmed.ncbi.nlm.nih.gov/21807894/

5. Okabe, Yasutaka, Sano, Teruyuki, Nagata, Shigekazu. 2009. Regulation of the innate immune response by threonine-phosphatase of Eyes absent. In Nature, 460, 520-4. doi:10.1038/nature08138. https://pubmed.ncbi.nlm.nih.gov/19561593/

6. Oka, Takafumi, Hikoso, Shungo, Yamaguchi, Osamu, Komuro, Issei, Otsu, Kinya. . Mitochondrial DNA that escapes from autophagy causes inflammation and heart failure. In Nature, 485, 251-5. doi:10.1038/nature10992. https://pubmed.ncbi.nlm.nih.gov/22535248/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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