C57BL/6NCya-Egfrem1/Cya
Common Name:
Egfr-KO
Product ID:
S-KO-01849
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Egfr-KO
Strain ID
KOCMP-13649-Egfr-B6N-VA
Gene Name
Product ID
S-KO-01849
Gene Alias
9030024J15Rik; Erbb; Errb1; Errp; Wa5; wa-2; wa2
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Egfrem1/Cya mice (Catalog S-KO-01849) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020329
NCBI RefSeq
NM_207655
Target Region
Exon 15~16
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Egfr, also known as the epidermal growth factor receptor, belongs to the ERBB family of tyrosine kinase receptors. Its signaling cascade is a key regulator in cell proliferation, differentiation, division, survival, and is associated with cancer development [1]. The activation of Egfr leads to autophosphorylation of receptor tyrosine kinase, initiating downstream signaling pathways involved in regulating cellular functions [5].
In non-small cell lung cancer (NSCLC), Egfr mutations are the second most common oncogenic driver event. Classical activating mutations like exon 19 deletions and the L858R point mutation are strong predictors for a good clinical response to Egfr tyrosine kinase inhibitors (Egfri). However, low-frequency mutations within exons 18-25 of the Egfr gene in NSCLC are associated with poorer responses to Egfri [2]. Moreover, in cancer, inappropriate activation of Egfr mainly results from amplification and point mutations at the genomic locus, transcriptional upregulation, or ligand overproduction [3]. Additionally, in NSCLC patients, resistance to tyrosine kinase inhibitors (TKIs) can occur through two central mechanisms: Egfr secondary mutations and bypass signaling activations [4].
In conclusion, Egfr is crucial in regulating normal cellular processes and is significantly involved in cancer development, especially in NSCLC. The study of Egfr in mouse models, though not explicitly detailed in the provided references, would likely further clarify its role in normal biology and disease. Understanding the functions of Egfr and its mutations in NSCLC can potentially lead to the development of more effective treatment strategies for patients with this disease.
References:
1. Sabbah, Dima A, Hajjo, Rima, Sweidan, Kamal. . Review on Epidermal Growth Factor Receptor (EGFR) Structure, Signaling Pathways, Interactions, and Recent Updates of EGFR Inhibitors. In Current topics in medicinal chemistry, 20, 815-834. doi:10.2174/1568026620666200303123102. https://pubmed.ncbi.nlm.nih.gov/32124699/
2. Harrison, Peter T, Vyse, Simon, Huang, Paul H. 2019. Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer. In Seminars in cancer biology, 61, 167-179. doi:10.1016/j.semcancer.2019.09.015. https://pubmed.ncbi.nlm.nih.gov/31562956/
3. Sigismund, Sara, Avanzato, Daniele, Lanzetti, Letizia. 2017. Emerging functions of the EGFR in cancer. In Molecular oncology, 12, 3-20. doi:10.1002/1878-0261.12155. https://pubmed.ncbi.nlm.nih.gov/29124875/
4. Liu, Qian, Yu, Shengnan, Zhao, Weiheng, Chu, Qian, Wu, Kongming. 2018. EGFR-TKIs resistance via EGFR-independent signaling pathways. In Molecular cancer, 17, 53. doi:10.1186/s12943-018-0793-1. https://pubmed.ncbi.nlm.nih.gov/29455669/
5. Singh, Davinder, Attri, Bhupinder Kumar, Gill, Rupinder Kaur, Bariwal, Jitender. . Review on EGFR Inhibitors: Critical Updates. In Mini reviews in medicinal chemistry, 16, 1134-66. doi:. https://pubmed.ncbi.nlm.nih.gov/26996617/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen