C57BL/6JCya-Eomesem1/Cya
Common Name:
Eomes-KO
Product ID:
S-KO-01887
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Eomes-KO
Strain ID
KOCMP-13813-Eomes-B6J-VB
Gene Name
Product ID
S-KO-01887
Gene Alias
TBR-2; Tbr2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eomesem1/Cya mice (Catalog S-KO-01887) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035020
NCBI RefSeq
NM_010136
Target Region
Exon 1~6
Size of Effective Region
~5.6 kb
Detailed Document
Overview of Gene Research
Eomes, also known as Eomesodermin, is a transcription factor that plays crucial roles in the development and function of various immune cells and in early embryonic development. In the immune system, it is involved in the differentiation and functional maintenance of natural killer (NK) cells, type 1 innate lymphoid cells (ILC1s), CD8+ T cells, and CD4+ T cells, and is associated with pathways related to immune responses against infections and tumors. In embryonic development, it participates in the specification of mesoderm and definitive endoderm [1,2,3,4,6,7]. Genetic models, such as gene knockout (KO) mouse models, have been valuable in studying Eomes.
In immune cell development, Eomes expression identifies the early bone marrow precursor to classical NK cells. Transfer of EomeshiNKneg cells into Rag2-/-Il2rg-/- recipients generated functional NK cells, while transfer of other ILC precursors did not, demonstrating that the NK and ILC1 lineages diverge early during development [1]. In CD4+ T cells, Eomes deletion protected against central nervous system (CNS) inflammation. Eomes is required for long-term maintenance of CNS-infiltrating CD4+ T cells, associated with sustained expression of genes involved in mitochondrial organization and functions [2]. In human NK cells, deleting T-BET and Eomes using Nuclease technology compromised in vivo antitumor response, normal proliferation, persistence, and cytokine responses. Eomes-deleted CD56bright NK cells acquired an innate lymphoid cell precursor-like profile [3]. In CD8+ T cells within the tumor microenvironment, Eomes-dependent loss of the co-activating receptor CD226 restrained anti-tumor functions and limited the efficacy of cancer immunotherapy [5].
In conclusion, Eomes is essential for the development and function of multiple immune cell types, with its dysregulation associated with various diseases, especially those related to immune-mediated inflammation and cancer. KO mouse models have been instrumental in revealing these functions, highlighting the significance of Eomes in understanding immune-related disease mechanisms and potentially guiding the development of new immunotherapeutic strategies.
References:
1. Liang, Zhitao, Anderson, Hope D, Locher, Veronica, McDonald, Benjamin D, Bendelac, Albert. 2024. Eomes expression identifies the early bone marrow precursor to classical NK cells. In Nature immunology, 25, 1172-1182. doi:10.1038/s41590-024-01861-6. https://pubmed.ncbi.nlm.nih.gov/38871999/
2. Joulia, Emeline, Michieletto, Michaël F, Agesta, Arantxa, Sarry, Jean-Emmanuel, Dejean, Anne S. 2024. Eomes-dependent mitochondrial regulation promotes survival of pathogenic CD4+ T cells during inflammation. In The Journal of experimental medicine, 221, . doi:10.1084/jem.20230449. https://pubmed.ncbi.nlm.nih.gov/38189779/
3. Wong, Pamela, Foltz, Jennifer A, Chang, Lily, Berrien-Elliott, Melissa M, Fehniger, Todd A. 2023. T-BET and EOMES sustain mature human NK cell identity and antitumor function. In The Journal of clinical investigation, 133, . doi:10.1172/JCI162530. https://pubmed.ncbi.nlm.nih.gov/37279078/
4. Geginat, Jens, Vasco, Chiara, Gruarin, Paola, Pagani, Massimiliano, Abrignani, Sergio. 2023. Eomesodermin-expressing type 1 regulatory (EOMES+ Tr1)-like T cells: Basic biology and role in immune-mediated diseases. In European journal of immunology, 53, e2149775. doi:10.1002/eji.202149775. https://pubmed.ncbi.nlm.nih.gov/36653901/
5. Weulersse, Marianne, Asrir, Assia, Pichler, Andrea C, Smyth, Mark J, Martinet, Ludovic. . Eomes-Dependent Loss of the Co-activating Receptor CD226 Restrains CD8+ T Cell Anti-tumor Functions and Limits the Efficacy of Cancer Immunotherapy. In Immunity, 53, 824-839.e10. doi:10.1016/j.immuni.2020.09.006. https://pubmed.ncbi.nlm.nih.gov/33053331/
6. Liao, Yue, Zheng, Yanling, Zhang, Ruizhi, Li, Xiaomin, Shen, Erxia. 2024. Regulatory roles of transcription factors T-bet and Eomes in group 1 ILCs. In International immunopharmacology, 143, 113229. doi:10.1016/j.intimp.2024.113229. https://pubmed.ncbi.nlm.nih.gov/39357208/
7. Schüle, Katrin M, Weckerle, Jelena, Probst, Simone, Schlägl, Inga-Marie, Arnold, Sebastian J. 2023. Eomes restricts Brachyury functions at the onset of mouse gastrulation. In Developmental cell, 58, 1627-1642.e7. doi:10.1016/j.devcel.2023.07.023. https://pubmed.ncbi.nlm.nih.gov/37633271/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen