C57BL/6JCya-Fhl1em1/Cya
Common Name
Fhl1-KO
Product ID
S-KO-02059
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14199-Fhl1-B6J-VA
When using this mouse strain in a publication, please cite “Fhl1-KO Mouse (Catalog S-KO-02059) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fhl1-KO
Strain ID
KOCMP-14199-Fhl1-B6J-VA
Gene Name
Product ID
S-KO-02059
Gene Alias
KyoT, SLIM, FHL-1, SLIM-1, RAM14-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000114769
NCBI RefSeq
NM_001077361
Target Region
Exon 3~8
Size of Effective Region
~5.3 kb
Overview of Gene Research
Fhl1, the four-and-a-half-LIM domain protein 1, has diverse functions. It is involved in multiple biological processes and is associated with various signaling pathways. Genetic models, especially knockout mouse models, are valuable for studying its functions. It has been linked to different disease conditions, highlighting its overall biological importance [1-3].
In gene knockout studies, FHL1-/-mice infected with chikungunya virus (CHIKV) or o'nyong-nyong virus (ONNV) showed reduced arthritis, myositis, immune infiltrates, and pro-inflammatory cytokine/chemokine outputs compared to wild-type mice [1]. This indicates that FHL1 promotes CHIKV and ONNV infection and pathogenesis. In glioblastoma, FHL1 overexpression promotes cell growth, migration, and invasion both in vivo and in vitro, while its silencing has the opposite effects, suggesting its role in promoting tumor malignancy [2]. In acute myeloid leukemia (AML), knockdown of FHL1 inhibited cell proliferation, induced cell cycle arrest, and promoted cell differentiation, and in vivo depletion of FHL1 inhibited tumor growth [3].
In conclusion, Fhl1 is involved in multiple biological processes and disease conditions. Gene knockout mouse models have been crucial in revealing its role in viral infections like those caused by CHIKV and ONNV, as well as in cancers such as glioblastoma and AML. These studies provide insights into potential therapeutic targets related to Fhl1-associated diseases.
References:
1. Ng, Wern Hann, Liu, Xiang, Ling, Zheng L, King, Nicholas J C, Mahalingam, Suresh. 2023. FHL1 promotes chikungunya and o'nyong-nyong virus infection and pathogenesis with implications for alphavirus vaccine design. In Nature communications, 14, 6605. doi:10.1038/s41467-023-42330-2. https://pubmed.ncbi.nlm.nih.gov/37884534/
2. Sun, Lili, Chen, Lili, Zhu, Hua, Chen, Clark C, Li, Ming. 2020. FHL1 promotes glioblastoma aggressiveness through regulating EGFR expression. In FEBS letters, 595, 85-98. doi:10.1002/1873-3468.13955. https://pubmed.ncbi.nlm.nih.gov/33053208/
3. Liu, Yabo, Li, Huibo, Zhao, Yanqiu, Fu, Jinyue, Fan, Shengjin. 2022. Targeting FHL1 impairs cell proliferation and differentiation of acute myeloid leukemia cells. In Biochemistry and cell biology = Biochimie et biologie cellulaire, 100, 301-308. doi:10.1139/bcb-2021-0507. https://pubmed.ncbi.nlm.nih.gov/35916339/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.