C57BL/6NCya-Gnat2em1/Cya
Common Name:
Gnat2-KO
Product ID:
S-KO-02276
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Gnat2-KO
Strain ID
KOCMP-14686-Gnat2-B6N-VA
Gene Name
Product ID
S-KO-02276
Gene Alias
Gnat-2; Gt-2; Hg1d; Tcalpha
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Gnat2em1/Cya mice (Catalog S-KO-02276) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058669
NCBI RefSeq
NM_008141
Target Region
Exon 2~9
Size of Effective Region
~8.2 kb
Detailed Document
Overview of Gene Research
GNAT2, also known as guanine nucleotide-binding protein subunit alpha transducin 2, is crucial for cone phototransduction in the retina. It is part of the phototransduction pathway in cone photoreceptor cells, which is essential for color vision and vision in bright light conditions [2,3,4].
In a Gnat2 knockout mouse line (Gnat2-/-), loss of Gnat2 expression led to the abolition of cone phototransduction. However, there was no loss of cones, disruption of the photoreceptor mosaic, or change in general retinal morphology up to at least 9 months of age. This indicates that Gnat2 is specifically required for cone function rather than cone survival. Retinal microglia and Müller glia were also distributed normally, suggesting that Gnat2 deficiency does not cause widespread retinal pathophysiology [4]. In humans, mutations in GNAT2 are associated with achromatopsia, a condition characterized by nystagmus, decreased visual acuity, light sensitivity, and variable color vision loss. The cone mosaic in GNAT2-associated achromatopsia is relatively well-preserved, potentially allowing for a wide therapeutic window for cone-directed interventions [1,3].
In conclusion, GNAT2 is essential for cone-mediated phototransduction in the retina. The Gnat2 knockout mouse model has been valuable in dissecting the role of GNAT2 in cone function, independent of photoreceptor degeneration. Understanding GNAT2's function is crucial for developing therapies for achromatopsia, a disease associated with its genetic mutations [1,3,4].
References:
1. Georgiou, Michalis, Singh, Navjit, Kane, Thomas, Carroll, Joseph, Michaelides, Michel. . Photoreceptor Structure in GNAT2-Associated Achromatopsia. In Investigative ophthalmology & visual science, 61, 40. doi:10.1167/iovs.61.3.40. https://pubmed.ncbi.nlm.nih.gov/32203983/
2. Hassall, Mark M, Barnard, Alun R, MacLaren, Robert E. 2017. Gene Therapy for Color Blindness. In The Yale journal of biology and medicine, 90, 543-551. doi:. https://pubmed.ncbi.nlm.nih.gov/29259520/
3. Georgiou, Michalis, Robson, Anthony G, Fujinami, Kaoru, Webster, Andrew R, Michaelides, Michel. 2024. Phenotyping and genotyping inherited retinal diseases: Molecular genetics, clinical and imaging features, and therapeutics of macular dystrophies, cone and cone-rod dystrophies, rod-cone dystrophies, Leber congenital amaurosis, and cone dysfunction syndromes. In Progress in retinal and eye research, 100, 101244. doi:10.1016/j.preteyeres.2024.101244. https://pubmed.ncbi.nlm.nih.gov/38278208/
4. Ronning, Kaitryn E, Allina, Gabriel Peinado, Miller, Eric B, Herrmann, Rolf, Burns, Marie E. 2018. Loss of cone function without degeneration in a novel Gnat2 knock-out mouse. In Experimental eye research, 171, 111-118. doi:10.1016/j.exer.2018.02.024. https://pubmed.ncbi.nlm.nih.gov/29518352/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen