C57BL/6NCya-Gnmtem1/Cya
Common Name
Gnmt-KO
Product ID
S-KO-02289
Backgroud
C57BL/6NCya
Strain ID
KOCMP-14711-Gnmt-B6N-VA
When using this mouse strain in a publication, please cite “Gnmt-KO Mouse (Catalog S-KO-02289) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gnmt-KO
Strain ID
KOCMP-14711-Gnmt-B6N-VA
Gene Name
Product ID
S-KO-02289
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000002846
NCBI RefSeq
NM_010321
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Overview of Gene Research
Gnmt, or glycine N-methyltransferase, is a key enzyme in one-carbon metabolism. It catalyzes the production of methylglycine (sarcosine) by taking a methyl group from S-adenosyl-L-methionine and methylating glycine, thus clearing methionine from the body [1,2]. This process is crucial for maintaining proper cellular methyl group availability, which is involved in various biological processes such as epigenetic modification [2].
In model organisms, Gnmt has significant impacts. In flies, Gnmt is required for reduced insulin/insulin-like growth factor 1 signaling and dietary restriction to fully extend lifespan [1]. In mice, Gnmt knockout models have shown that Gnmt deficiency has high penetrance in inducing the development of steatohepatitis and hepatocellular carcinoma [2]. Children with Gnmt mutation have elevated liver transaminases, and Gnmt knockout mice develop liver injury, fibrosis, and HCC, indicating that a normal hepatic S-adenosylmethionine (AdoMet) level, which is regulated by Gnmt, is necessary to maintain liver health [3].
In conclusion, Gnmt is essential for regulating methionine metabolism and maintaining proper methyl group availability. Model-based research, especially Gnmt knockout mouse models, has revealed its crucial role in liver-related diseases such as steatohepatitis, liver fibrosis, and hepatocellular carcinoma, highlighting its potential as a therapeutic target and biomarker in these disease areas [2,3].
References:
1. Johnson, Adiv A, Cuellar, Trinna L. 2023. Glycine and aging: Evidence and mechanisms. In Ageing research reviews, 87, 101922. doi:10.1016/j.arr.2023.101922. https://pubmed.ncbi.nlm.nih.gov/37004845/
2. Yen, Chia-Hung, Lin, Yu-Ting, Chen, Heng-Li, Chen, Shih-Yin, Chen, Yi-Ming Arthur. 2012. The multi-functional roles of GNMT in toxicology and cancer. In Toxicology and applied pharmacology, 266, 67-75. doi:10.1016/j.taap.2012.11.003. https://pubmed.ncbi.nlm.nih.gov/23147572/
3. Lu, Shelly C, Mato, José M. . S-adenosylmethionine in liver health, injury, and cancer. In Physiological reviews, 92, 1515-42. doi:10.1152/physrev.00047.2011. https://pubmed.ncbi.nlm.nih.gov/23073625/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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