C57BL/6JCya-Gpamem1/Cya
Common Name:
Gpam-KO
Product ID:
S-KO-02300
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Gpam-KO
Strain ID
KOCMP-14732-Gpam-B6J-VA
Gene Name
Product ID
S-KO-02300
Gene Alias
GPAT; GPAT-1; GPAT1; P90
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpamem1/Cya mice (Catalog S-KO-02300) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061856
NCBI RefSeq
NM_008149
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
GPAM, also known as glycerol-3-phosphate acyltransferase, mitochondrial, is a key enzyme. It catalyzes an essential step in the biosynthesis of glycerolphospholipids and triacylglycerol [4]. As a rate-limiting enzyme in the lysophosphatidic acid (LPA) synthesis pathway, it converts glycerol-3-phosphate to LPA [1]. Genetic models, such as gene knockout models, are valuable for studying its functions.
In acute myeloid leukemia (AML), GPAM is highly expressed. Silencing GPAM or using a GPAM-inhibitor (FSG67) significantly impairs AML cell propagation. This occurs through the induction of mitochondrial fission, suppression of oxidative phosphorylation, and elevation of reactive oxygen species. Notably, FSG67 administration does not affect normal human hematopoiesis in vivo, suggesting GPAM-mediated LPA synthesis is a critical metabolic mechanism regulating mitochondrial dynamics in AML [1]. In non-alcoholic fatty liver disease (NAFLD), rare, protective, predicted loss-of-function variants in GPAM have been identified, highlighting it as a potential drug target [2]. In cholangiocarcinoma (CCA), the lnc-PKD2-2-3/miR-328/GPAM ceRNA network promotes CCA cell proliferation, invasion, and 5-FU chemoresistance [3].
In conclusion, GPAM plays essential roles in lipid-related metabolic pathways. Its study using gene knockout models has revealed its significance in diseases like AML, NAFLD, and CCA. Understanding GPAM's functions provides potential therapeutic targets for these diseases.
References:
1. Irifune, Hidetoshi, Kochi, Yu, Miyamoto, Toshihiro, Akashi, Koichi, Kikushige, Yoshikane. 2023. GPAM mediated lysophosphatidic acid synthesis regulates mitochondrial dynamics in acute myeloid leukemia. In Cancer science, 114, 3247-3258. doi:10.1111/cas.15835. https://pubmed.ncbi.nlm.nih.gov/37197765/
2. Sveinbjornsson, Gardar, Ulfarsson, Magnus O, Thorolfsdottir, Rosa B, Holm, Hilma, Stefansson, Kari. 2022. Multiomics study of nonalcoholic fatty liver disease. In Nature genetics, 54, 1652-1663. doi:10.1038/s41588-022-01199-5. https://pubmed.ncbi.nlm.nih.gov/36280732/
3. Zhang, Lei, Ma, Donglai, Li, Fujun, Sun, Dongsheng, Zeng, Zhaolin. 2022. Lnc-PKD2-2-3/miR-328/GPAM ceRNA Network Induces Cholangiocarcinoma Proliferation, Invasion and 5-FU Chemoresistance. In Frontiers in oncology, 12, 871281. doi:10.3389/fonc.2022.871281. https://pubmed.ncbi.nlm.nih.gov/35965521/
4. Sun, Chuanbo, Li, Bing, Yang, Miaomiao, Wang, Jichang, Hu, Hao. 2021. Generation of GPAM knockout human embryonic stem cell line SYSUe-008-A using Nuclease technology. In Stem cell research, 53, 102303. doi:10.1016/j.scr.2021.102303. https://pubmed.ncbi.nlm.nih.gov/33831647/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen