C57BL/6NCya-Htr3aem1/Cya
Common Name
Htr3a-KO
Product ID
S-KO-02542
Backgroud
C57BL/6NCya
Strain ID
KOCMP-15561-Htr3a-B6N-VA
When using this mouse strain in a publication, please cite “Htr3a-KO Mouse (Catalog S-KO-02542) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Htr3a-KO
Strain ID
KOCMP-15561-Htr3a-B6N-VA
Gene Name
Product ID
S-KO-02542
Gene Alias
5-HT3, 5-HT3A, 5-HT3R
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000003826
NCBI RefSeq
NM_013561
Target Region
Exon 2~4
Size of Effective Region
~2.5 kb
Overview of Gene Research
Htr3a, the main subunit of 5-hydroxytryptamine 3 (5-HT3) receptors, is a ligand-gated ion channel gene. 5-HT3 receptors are involved in multiple physiological processes. They are part of the serotonin pathway and play important roles in the central and peripheral nervous systems [2,4].
In knockout mouse models, Htr3a knockout (KO) mice showed autistic-like behaviors, impaired memory, and attenuated seizure susceptibility. Enhanced GABAergic transmission in pyramidal neurons and up-regulation of NMDAR were observed, suggesting NMDAR system could be a potential therapeutic target for autism related to HTR3A deficiency [1]. Conditional knockout of Setdb1 in neural progenitor cells led to up-regulation of Htr3a expression, increased number and excitability of Htr3a+ interneurons in the cortex, and Setdb1-Gad2-cKO mice showed anxiety-and depressive-like behaviors, which were partially reversed by a 5-HT3 receptor antagonist [3].
In conclusion, Htr3a is crucial for normal neural function as demonstrated by KO and conditional knockout mouse models. These models have revealed its role in conditions such as autism, anxiety, and depression, providing insights into potential therapeutic targets for these diseases.
References:
1. Huang, Lang, Wang, Jing, Liang, Guanmei, Zhong, Mei, Yang, Xinping. 2021. Upregulated NMDAR-mediated GABAergic transmission underlies autistic-like deficits in Htr3a knockout mice. In Theranostics, 11, 9296-9310. doi:10.7150/thno.60531. https://pubmed.ncbi.nlm.nih.gov/34646371/
2. Tone, Mitsuyo, Tahara, Shinichiro, Nojima, Satoshi, Okuzaki, Daisuke, Morii, Eiichi. 2020. HTR3A is correlated with unfavorable histology and promotes proliferation through ERK phosphorylation in lung adenocarcinoma. In Cancer science, 111, 3953-3961. doi:10.1111/cas.14592. https://pubmed.ncbi.nlm.nih.gov/32736413/
3. Li, Jiaqi, Zheng, Shenghui, Dong, Yuhao, Xiao, Lei, Jiang, Yan. 2022. Histone Methyltransferase SETDB1 Regulates the Development of Cortical Htr3a-Positive Interneurons and Mood Behaviors. In Biological psychiatry, 93, 279-290. doi:10.1016/j.biopsych.2022.08.021. https://pubmed.ncbi.nlm.nih.gov/36335068/
4. Niesler, Beate, Kapeller, Johannes, Hammer, Christian, Rappold, Gudrun. . Serotonin type 3 receptor genes: HTR3A, B, C, D, E. In Pharmacogenomics, 9, 501-4. doi:10.2217/14622416.9.5.501. https://pubmed.ncbi.nlm.nih.gov/18466097/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.