C57BL/6JCya-Itga2em1/Cya
Common Name:
Itga2-KO
Product ID:
S-KO-02688
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Itga2-KO
Strain ID
KOCMP-16398-Itga2-B6J-VA
Gene Name
Product ID
S-KO-02688
Gene Alias
CD49B; DX5; GPIa
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itga2em1/Cya mice (Catalog S-KO-02688) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056117
NCBI RefSeq
NM_008396
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Itga2, also known as CD49b or VLA-2, is the alpha subunit of the transmembrane collagen receptor integrin alpha-2/beta-1. It plays a crucial role in cell-matrix interactions by binding to collagens and related proteins. The integrin-mediated signaling pathways it is involved in can impact various biological processes such as cell adhesion, migration, proliferation, and apoptosis. These functions are essential for normal tissue development and homeostasis [5].
In cancer research, multiple studies have revealed its significance. In pancreatic ductal adenocarcinoma (PDAC), overexpression of Itga2 inhibits the non-homologous end joining (NHEJ) pathway of DNA repair, conferring sensitivity to radiotherapy. This suggests that Itga2 expression could be an indicator for radiotherapy and DNA damage reagents in PDAC patients [1]. In glioblastoma, Itga2 was identified as a novel molecular target. Antibody blockade of Itga2 impedes cell migration, and ITGA2-antibody-directed liposomes can selectively deliver doxorubicin to glioblastoma cells, improving antitumor efficacy [2]. In pancreatic cancer, high expression of Itga2 affects the expression of MET, PD-L1, CD4, and CD8, influencing the immune microenvironment. Blocking Itga2 inhibits pancreatic cancer cell proliferation and invasion [3]. In colorectal cancer, Itga2 is associated with 5-fluorouracil resistance, and knocking down Itga2 can restore sensitivity to 5-FU, with selumetinib enhancing this effect [4]. In pancreatic cancer, Itga2 activates the cytosolic DNA-sensing pathway and promotes STING expression by inducing DNMT1 degradation [5]. In esophageal squamous cell carcinoma, Itga2 overexpression promotes tumor aggression via the FAK/AKT signaling pathway [6]. In intrahepatic cholangiocarcinoma, elevated Itga2 expression promotes collagen type I-induced clonogenic growth [7]. In thyroid carcinoma, hypomethylation-mediated overexpression of Itga2 stimulates cell invasion and migration [8]. In ovarian cancer, overexpressed Itga2 contributes to paclitaxel resistance through the activation of the AKT/FoxO1 pathway [9].
In conclusion, Itga2 is a key gene involved in cell-matrix interactions and has a significant impact on various biological processes. Through functional studies, especially in cancer models, it has been shown to play diverse roles in cancer progression, including affecting DNA repair, drug resistance, immune microenvironment, and cell invasion and migration. These findings suggest that targeting Itga2 could be a potential therapeutic strategy in multiple cancer types.
References:
1. Zhou, Chen, Li, Shoukang, Bin, Kaijian, Wu, Heshui, Zhou, Yingke. 2022. ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer. In Cancer letters, 547, 215855. doi:10.1016/j.canlet.2022.215855. https://pubmed.ncbi.nlm.nih.gov/35998796/
2. Guo, Peng, Moses-Gardner, Alexander, Huang, Jing, Smith, Edward R, Moses, Marsha A. 2019. ITGA2 as a potential nanotherapeutic target for glioblastoma. In Scientific reports, 9, 6195. doi:10.1038/s41598-019-42643-7. https://pubmed.ncbi.nlm.nih.gov/30996239/
3. Jin, Liquan, Duan, Yaoqiang, Li, Xiaoxi, Chen, Yiming, Tan, Yunbo. 2023. High expression ITGA2 affects the expression of MET, PD-L1, CD4 and CD8 with the immune microenvironment in pancreatic cancer patients. In Frontiers in immunology, 14, 1209367. doi:10.3389/fimmu.2023.1209367. https://pubmed.ncbi.nlm.nih.gov/37881431/
4. Qin, Jian, Hu, Shangshang, Lou, Jinwei, Pan, Yuqin, Wang, Shukui. 2024. Selumetinib overcomes ITGA2-induced 5-fluorouracil resistance in colorectal cancer. In International immunopharmacology, 137, 112487. doi:10.1016/j.intimp.2024.112487. https://pubmed.ncbi.nlm.nih.gov/38889513/
5. Meng, Junpeng, Cai, Hongkun, Sun, Yan, Wu, Heshui, Ren, Dianyun. 2022. ITGA2 induces STING expression in pancreatic cancer by inducing DNMT1 degradation. In Cellular oncology (Dordrecht, Netherlands), 45, 1421-1434. doi:10.1007/s13402-022-00731-3. https://pubmed.ncbi.nlm.nih.gov/36331797/
6. Huang, Wei, Zhu, Ju, Shi, Haoming, Wu, Qingchen, Zhang, Cheng. 2021. ITGA2 Overexpression Promotes Esophageal Squamous Cell Carcinoma Aggression via FAK/AKT Signaling Pathway. In OncoTargets and therapy, 14, 3583-3596. doi:10.2147/OTT.S302028. https://pubmed.ncbi.nlm.nih.gov/34113124/
7. Rattanasinchai, Chotirat, Navasumrit, Panida, Ruchirawat, Mathuros. 2022. Elevated ITGA2 expression promotes collagen type I-induced clonogenic growth of intrahepatic cholangiocarcinoma. In Scientific reports, 12, 22429. doi:10.1038/s41598-022-26747-1. https://pubmed.ncbi.nlm.nih.gov/36575207/
8. Chen, Hong, Zhang, Chunying, Li, Yanbing, Chen, Chunyou. 2022. Hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration of thyroid carcinoma. In Histology and histopathology, 38, 787-796. doi:10.14670/HH-18-552. https://pubmed.ncbi.nlm.nih.gov/36420922/
9. Ma, Linlin, Sun, Yan, Li, Dan, Jin, Xin, Ren, Dianyun. 2020. Overexpressed ITGA2 contributes to paclitaxel resistance by ovarian cancer cells through the activation of the AKT/FoxO1 pathway. In Aging, 12, 5336-5351. doi:10.18632/aging.102954. https://pubmed.ncbi.nlm.nih.gov/32202508/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen