C57BL/6NCya-Cd47em1/Cya
Common Name:
Cd47-KO
Product ID:
S-KO-02707
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cd47-KO
Strain ID
KOCMP-16423-Cd47-B6N-VA
Gene Name
Product ID
S-KO-02707
Gene Alias
9130415E20Rik; B430305P08Rik; IAP; Itgp
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cd47em1/Cya mice (Catalog S-KO-02707) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000084838
NCBI RefSeq
NM_010581
Target Region
Exon 2~8
Size of Effective Region
~30.4 kb
Detailed Document
Overview of Gene Research
Cd47, or Cluster of Differentiation 47, is a protein expressed on both healthy and cancer cells. It plays a pivotal role in regulating the cytotoxic activity of myeloid cells by delivering a “don't eat me signal” when it binds to the Signal-regulatory protein alpha (SIRPα) receptor on myeloid cells. This interaction is part of a balance of signals transmitted through inhibitory and activating receptors, which is crucial for physiological tissue homeostasis [1].
In cancer research, anti-Cd47 antibody-based therapies have been investigated. In syngeneic immunocompetent mouse tumor models, the therapeutic effects of Cd47 blockade were found to depend on dendritic cell-mediated cross-priming of T cell responses rather than macrophage phagocytosis as previously thought, and were abrogated in T cell-deficient mice. Also, the antitumor effects required the cytosolic DNA sensor STING in CD11c + cells [3]. In glioblastoma, enhanced fatty acid metabolism promotes aggressive growth with Cd47-mediated immune evasion, and targeting the FAO-Cd47 axis may improve radioimmunotherapy [2].
In conclusion, Cd47 is essential in regulating the immune response, especially the interaction between myeloid cells and other cells. Studies using mouse models have revealed its significance in cancer, such as in promoting tumor immune evasion and influencing the efficacy of immunotherapies. Understanding Cd47 could potentially lead to the development of more effective cancer treatments.
References:
1. Logtenberg, Meike E W, Scheeren, Ferenc A, Schumacher, Ton N. . The CD47-SIRPα Immune Checkpoint. In Immunity, 52, 742-752. doi:10.1016/j.immuni.2020.04.011. https://pubmed.ncbi.nlm.nih.gov/32433947/
2. Jiang, Nian, Xie, Bowen, Xiao, Wenwu, Chen, Hong-Wu, Li, Jian Jian. 2022. Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion. In Nature communications, 13, 1511. doi:10.1038/s41467-022-29137-3. https://pubmed.ncbi.nlm.nih.gov/35314680/
3. Liu, Xiaojuan, Pu, Yang, Cron, Kyle, Fu, Yang-Xin, Xu, Meng Michelle. 2015. CD47 blockade triggers T cell-mediated destruction of immunogenic tumors. In Nature medicine, 21, 1209-15. doi:10.1038/nm.3931. https://pubmed.ncbi.nlm.nih.gov/26322579/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen