C57BL/6JCya-Lgals9em1/Cya
Common Name
Lgals9-KO
Product ID
S-KO-02886
Backgroud
C57BL/6JCya
Strain ID
KOCMP-16859-Lgals9-B6J-VA
When using this mouse strain in a publication, please cite “Lgals9-KO Mouse (Catalog S-KO-02886) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Lgals9-KO
Strain ID
KOCMP-16859-Lgals9-B6J-VA
Gene Name
Product ID
S-KO-02886
Gene Alias
LGALS35, Lgals5, gal-9, galectin-9
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000108269
NCBI RefSeq
NM_010708
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Overview of Gene Research
Lgals9, also known as Galectin-9, is a member of the β-galactosidase family and plays a crucial role in immune regulation. It is involved in multiple pathways, such as binding to cell-surface glycoprotein CD44 and T cell immunoglobulin and mucin domain 3 (TIM-3), which are associated with immune cell apoptosis, activation, and immune-related disease processes [2,3,4].
In a murine stroke model, extracellular vesicle-mediated Lgals9 delivery improved long-term functional recovery, and knockdown of Cd44 reversed these therapeutic effects, inhibiting oligodendrocyte differentiation and remyelination, suggesting Lgals9-CD44 as a critical signaling pathway after ischemic injury [1]. In preeclampsia, galectin-9 from trophoblasts activates CD11chigh decidual macrophages via CD44 binding, suppressing uterine spiral artery remodeling. Galectin-9 blockade or macrophage-specific CD44 deletion prevents preeclampsia-like phenotypes in mouse models [3].
In conclusion, Lgals9 is essential in immune regulation and has a significant impact on diseases like stroke and preeclampsia. The use of gene-knockout or conditional-knockout mouse models in these studies has revealed its role in specific biological processes, providing valuable insights into potential treatment strategies for these diseases.
References:
1. Han, Bing, Zhou, Shunheng, Zhang, Yuan, Jiang, Wei, Yao, Honghong. 2024. Integrating spatial and single-cell transcriptomics to characterize the molecular and cellular architecture of the ischemic mouse brain. In Science translational medicine, 16, eadg1323. doi:10.1126/scitranslmed.adg1323. https://pubmed.ncbi.nlm.nih.gov/38324639/
2. Zhao, Lizhen, Cheng, Shaoyun, Fan, Lin, Zhang, Bei, Xu, Shengwei. 2021. TIM-3: An update on immunotherapy. In International immunopharmacology, 99, 107933. doi:10.1016/j.intimp.2021.107933. https://pubmed.ncbi.nlm.nih.gov/34224993/
3. Li, Yanhong, Sang, Yifei, Chang, Yunjian, Wu, Ligang, Du, Meirong. 2024. A Galectin-9-Driven CD11chigh Decidual Macrophage Subset Suppresses Uterine Vascular Remodeling in Preeclampsia. In Circulation, 149, 1670-1688. doi:10.1161/CIRCULATIONAHA.123.064391. https://pubmed.ncbi.nlm.nih.gov/38314577/
4. Yang, Riyao, Sun, Linlin, Li, Ching-Fei, Yu, Dihua, Hung, Mien-Chie. 2021. Galectin-9 interacts with PD-1 and TIM-3 to regulate T cell death and is a target for cancer immunotherapy. In Nature communications, 12, 832. doi:10.1038/s41467-021-21099-2. https://pubmed.ncbi.nlm.nih.gov/33547304/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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