C57BL/6NCya-Loxl1em1/Cya
Common Name:
Loxl1-KO
Product ID:
S-KO-02905
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Loxl1-KO
Strain ID
KOCMP-16949-Loxl1-B6N-VA
Gene Name
Product ID
S-KO-02905
Gene Alias
Loxl
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Loxl1em1/Cya mice (Catalog S-KO-02905) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061799
NCBI RefSeq
NM_010729
Target Region
Exon 1
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Loxl1, or lysyl oxidase-like 1, is a matrix cross-linking enzyme crucial for elastic fiber formation [1,2]. It may be involved in multiple biological processes, and its associated pathways likely relate to extracellular matrix remodeling. Genetic models, such as gene-edited zebrafish, can be valuable for studying its functions [2].
In zebrafish, loxl1 deficiency caused zonular bundling disorders in juveniles and accumulation of pearl-like particles in adults, along with thinning or loss of the elastic lamina in Bruch's membrane, mimicking some ocular manifestations of exfoliation syndrome (XFS) [2]. In human studies, certain genetic variants of LOXL1 confer risk for XFS, but are not the sole causative factor as many genetically affected individuals do not develop the disease or subsequent glaucoma (XFG) [1]. In XFG patient-derived cells, LOXL1 protein undergoes autophagic clearance, suggesting aggregation [1].
In conclusion, Loxl1 is essential for elastic fiber formation and is implicated in XFS. The loxl1-deficient zebrafish model provides insights into the role of Loxl1 in XFS-related ocular pathologies, highlighting its significance in understanding the disease mechanism.
References:
1. Bernstein, Audrey M, Ritch, Robert, Wolosin, J Mario. 2019. LOXL1 folding in exfoliation glaucoma. In Advances in protein chemistry and structural biology, 118, 273-288. doi:10.1016/bs.apcsb.2019.09.005. https://pubmed.ncbi.nlm.nih.gov/31928728/
2. Zhang, Min, Sun, Shaoyang, Wang, Lei, Chen, Zexu, Jiang, Yongxiang. 2021. Zonular defects in loxl1-deficient zebrafish. In Clinical & experimental ophthalmology, 50, 62-73. doi:10.1111/ceo.14017. https://pubmed.ncbi.nlm.nih.gov/34585825/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen