C57BL/6NCya-Tlr7em1/Cya
Common Name:
Tlr7-KO
Product ID:
S-KO-02979
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tlr7-KO
Strain ID
KOCMP-170743-Tlr7-B6N-VA
Gene Name
Product ID
S-KO-02979
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tlr7em1/Cya mice (Catalog S-KO-02979) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060719
NCBI RefSeq
NM_133211
Target Region
Exon 3
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Tlr7, a Toll-like receptor, is a key pattern recognition receptor in the innate immune system. It recognizes pathogen-derived single-stranded RNA (ssRNA), initiating antiviral immunity and triggering the release of pro-inflammatory cytokines or type-I interferons, which is crucial for immunoregulation [1,3]. It operates via the MyD88-dependent signaling pathway [1].
In lupus, hyperactive Tlr7 signaling is a major driver. In TLR9-deficient MRL/lpr mice (a lupus-prone model), B cell-specific Tlr7 deficiency greatly improved disease, suggesting B cell-intrinsic Tlr7 expression is important for severe lupus development. This indicates a cis-regulatory interaction between protective TLR9 and pathogenic Tlr7 within the B cell compartment [4]. In rosacea, TLR7 promotes skin inflammation by activating the NFκB-mTORC1 axis. Silencing TLR7 in LL37-induced rosacea-like mouse models prevented the development of skin inflammation [2]. In pustular psoriasis, the TLR7-MyD88-DC-CXCL16 axis activates neutrophils to elicit an inflammatory response. In imiquimod-induced psoriasis-like skin lesions in mice, TLR7 influenced DC release of CXCL16 and neutrophil pro-inflammatory effects by interfering with the MyD88 signaling pathway [5].
In conclusion, Tlr7 is essential for antiviral immunity and immunoregulation. Its study using gene-knockout (KO) or conditional-knockout (CKO) mouse models has revealed its significance in autoimmune diseases such as lupus, rosacea, and pustular psoriasis. These models help in understanding the underlying molecular mechanisms, providing potential therapeutic targets for these diseases.
References:
1. Diebold, Sandra S, Kaisho, Tsuneyasu, Hemmi, Hiroaki, Akira, Shizuo, Reis e Sousa, Caetano. 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. In Science (New York, N.Y.), 303, 1529-31. doi:. https://pubmed.ncbi.nlm.nih.gov/14976261/
2. Huang, Yaqun, Liu, Da, Chen, Mengting, Liu, Fangfen, Xiao, Wenqin. 2023. TLR7 promotes skin inflammation via activating NFκB-mTORC1 axis in rosacea. In PeerJ, 11, e15976. doi:10.7717/peerj.15976. https://pubmed.ncbi.nlm.nih.gov/37780385/
3. Zheng, Haoyang, Wu, Peiyang, Bonnet, Pierre-Antoine. 2023. Recent Advances on Small-Molecule Antagonists Targeting TLR7. In Molecules (Basel, Switzerland), 28, . doi:10.3390/molecules28020634. https://pubmed.ncbi.nlm.nih.gov/36677692/
4. Cosgrove, Haylee A, Gingras, Sebastien, Kim, Minjung, Tilstra, Jeremy S, Shlomchik, Mark J. 2023. B cell-intrinsic TLR7 expression drives severe lupus in TLR9-deficient mice. In JCI insight, 8, . doi:10.1172/jci.insight.172219. https://pubmed.ncbi.nlm.nih.gov/37606042/
5. Lu, Jiajing, Zhong, Xiaoyuan, Guo, Chunyuan, Zhou, Jing, Shi, Yuling. 2023. TLR7-MyD88-DC-CXCL16 axis results neutrophil activation to elicit inflammatory response in pustular psoriasis. In Cell death & disease, 14, 315. doi:10.1038/s41419-023-05815-y. https://pubmed.ncbi.nlm.nih.gov/37160878/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen