C57BL/6NCya-Mstnem1/Cya
Common Name:
Mstn-KO
Product ID:
S-KO-03256
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mstn-KO
Strain ID
KOCMP-17700-Mstn-B6N-VA
Gene Name
Product ID
S-KO-03256
Gene Alias
Cmpt; Gdf8
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mstnem1/Cya mice (Catalog S-KO-03256) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027269
NCBI RefSeq
NM_010834
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Myostatin (MSTN), also known as GDF-8, is a protein belonging to the transforming growth factor-β superfamily. It is a negative regulator of skeletal muscle growth and development, inhibiting the proliferation of myoblasts. Mutations in MSTN can lead to skeletal muscle hyperplasia and hypertrophy, resulting in the "double-muscle" phenotype, which is of great significance for improving livestock meat production and has potential implications in treating muscle-related diseases in humans [1,2].
Mutant MSTN gene in cloned goats led to a "double-muscle" phenotype, with muscle fibers tightly arranged and thick, and higher monthly body weight compared to wild-type goats [1]. In sheep, a MSTNDel73C mutation with FGF5 knockout produced a dominant "double-muscle" phenotype, promoting skeletal muscle myofiber hyperplasia through the MEK-ERK-FOSL1 axis [2]. In Hu sheep, MSTN-edited animals showed a double-muscled phenotype, higher body weight at 3 and 4 months old, and enhanced AKT and suppressed ERK1/2 signaling in the gluteus muscle [3].
In conclusion, MSTN is a crucial negative regulator of skeletal muscle growth. Gene-edited animal models with MSTN mutations, such as goats, sheep, and pigs, have demonstrated its role in enhancing muscle mass and growth. These findings have implications for livestock breeding to improve meat production and potentially for developing treatments for muscle-loss-related human diseases [1,2,3].
References:
1. Song, Shao-Zheng, He, Zheng-Yi, Cheng, Yong, Zhang, Ting, Li, Dan. . MSTN modification in goat mediated by TALENs and performance analysis. In Yi chuan = Hereditas, 44, 531-542. doi:10.16288/j.yczz.22-011. https://pubmed.ncbi.nlm.nih.gov/35729101/
2. Chen, Ming-Ming, Zhao, Yue, Yu, Kun, Lian, Di, Lian, Zheng-Xing. 2024. A MSTNDel73C mutation with FGF5 knockout sheep by Nuclease technology promotes skeletal muscle myofiber hyperplasia. In eLife, 12, . doi:10.7554/eLife.86827. https://pubmed.ncbi.nlm.nih.gov/39365728/
3. Guo, Rihong, Wang, Huili, Meng, Chunhua, Zhong, Jifeng, Cao, Shaoxian. 2023. Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR. In Genes, 14, . doi:10.3390/genes14061216. https://pubmed.ncbi.nlm.nih.gov/37372396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen