C57BL/6NCya-Mthfrem1/Cya
Common Name:
Mthfr-KO
Product ID:
S-KO-03273
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mthfr-KO
Strain ID
KOCMP-17769-Mthfr-B6N-VA
Gene Name
Product ID
S-KO-03273
Gene Alias
-
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mthfrem1/Cya mice (Catalog S-KO-03273) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069604
NCBI RefSeq
NM_001161798
Target Region
Exon 3~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mthfr, or 5,10-Methylenetetrahydrofolate reductase, is a key enzyme in the folate metabolism pathway, which is integral for cell metabolism, especially in DNA, RNA, and protein methylation [1]. It is mapped on chromosome 1 at the end of the short arm (1p36.6). The enzyme is vital for maintaining methionine and homocysteine balance within cells, thus ensuring cellular homeostasis, and is involved in the one-carbon cycle, which includes methionine and folate metabolism and nucleic acid synthesis [2].
The Mthfr C677T polymorphism, located at exon 4, is associated with various diseases such as vascular diseases, cancers, neurological disorders, diabetes, psoriasis, and more [1]. This polymorphism results in an amino acid change from alanine to valine at codon 222, reducing the enzyme's activity. Homozygous mutated subjects have higher homocysteine levels, and heterozygous mutated subjects have mildly raised homocysteine levels compared to non-mutated controls [1]. Hyperhomocysteinemia is an emerging risk factor for cardiovascular diseases [1]. Additionally, Mthfr 1298A>C substitution shows an association with recurrent pregnancy loss in a population-specific manner, being a strong risk factor in Caucasian populations [3]. The C677T polymorphism of Mthfr has also been associated with an increased risk of rheumatoid arthritis, especially in African and Asian populations under certain genotype models [4], and with neural tube defects, particularly among Caucasians and Asians [5].
In conclusion, Mthfr is crucial for folate metabolism, methionine-homocysteine balance, and the one-carbon cycle, with significant implications for multiple biological processes and disease conditions. The study of Mthfr gene polymorphisms, especially C677T and 1298A>C, through genetic models can help understand its role in various diseases, providing potential insights for prevention and treatment strategies [1,2,3,4,5].
References:
1. Liew, Siaw-Cheok, Gupta, Esha Das. 2014. Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism: epidemiology, metabolism and the associated diseases. In European journal of medical genetics, 58, 1-10. doi:10.1016/j.ejmg.2014.10.004. https://pubmed.ncbi.nlm.nih.gov/25449138/
2. Raghubeer, Shanel, Matsha, Tandi E. 2021. Methylenetetrahydrofolate (MTHFR), the One-Carbon Cycle, and Cardiovascular Risks. In Nutrients, 13, . doi:10.3390/nu13124562. https://pubmed.ncbi.nlm.nih.gov/34960114/
3. Mehta, Poonam, Vishvkarma, Rahul, Singh, Kiran, Rajender, Singh. 2021. MTHFR 1298A>C Substitution is a Strong Candidate for Analysis in Recurrent Pregnancy Loss: Evidence from 14,289 Subjects. In Reproductive sciences (Thousand Oaks, Calif.), 29, 1039-1053. doi:10.1007/s43032-021-00530-5. https://pubmed.ncbi.nlm.nih.gov/33742421/
4. Bagheri-Hosseinabadi, Zahra, Imani, Danyal, Yousefi, Hassan, Abbasifard, Mitra. 2020. MTHFR gene polymorphisms and susceptibility to rheumatoid arthritis: a meta-analysis based on 16 studies. In Clinical rheumatology, 39, 2267-2279. doi:10.1007/s10067-020-05031-5. https://pubmed.ncbi.nlm.nih.gov/32170488/
5. Tabatabaei, Razieh Sadat, Fatahi-Meibodi, Neda, Meibodi, Bahare, Karimi-Zarchi, Mojgan, Neamatzadeh, Hossein. 2020. Association of Fetal MTHFR C677T Polymorphism with Susceptibility to Neural Tube Defects: A Systematic Review and Update Meta-Analysis. In Fetal and pediatric pathology, 41, 225-241. doi:10.1080/15513815.2020.1775734. https://pubmed.ncbi.nlm.nih.gov/32536242/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen