C57BL/6JCya-Muc2em1/Cya
Common Name:
Muc2-KO
Product ID:
S-KO-03285
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Muc2-KO
Strain ID
KOCMP-17831-Muc2-B6J-VB
Gene Name
Product ID
S-KO-03285
Gene Alias
2010015E03Rik; MCM; wnn
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Muc2em1/Cya mice (Catalog S-KO-03285) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000185406
NCBI RefSeq
NM_023566
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
MUC2, a subtype of secretory mucins, is produced by goblet cells and is the major macromolecular component of mucus in the gut mucosa [2]. Its primary function is to form a protective mucus barrier between epithelial surfaces and the gut lumen, preventing direct contact between intestinal bacteria and colonic epithelial cells, thus playing a crucial role in gut barrier function and homeostasis [1,2,4,5]. The production of MUC2 is regulated by various bioactive molecules, signaling pathways, and gut microbiota, forming a complex regulatory network [2].
In ulcerative colitis (UC), bacterial factors related to the MUC2 mucus barrier are involved in dietary pattern response, MUC2 mucus barrier dysfunction, contact stimulation with colonic epithelial cells, and mucosal and submucosal inflammation [1]. In colorectal cancer (CRC), MUC2 disruption is associated with the disease, and its expression is regulated by various modulators, being related to immunomodulation, tumor immunity, and microbiota regulation [3]. In breast cancer, MUC2 affects cancer cell proliferation, response to chemotherapy, and metastasis [7]. Also, in pancreatic neoplasia, MUC2 is rarely detectable in aggressive tumors but commonly expressed in indolent ones, suggesting potential diagnostic and prognostic value [6]. Additionally, MUC2 gene methylation detection in feces and blood shows promise as a new screening and early-diagnosis method for pancreatic cancer [8].
In summary, MUC2 is essential for maintaining gut barrier function and homeostasis. Its dysregulation is associated with multiple diseases, including UC, CRC, breast cancer, pancreatic neoplasia, and pancreatic cancer. Research on MUC2 using genetic models helps in understanding the underlying mechanisms of these diseases, potentially leading to new therapeutic strategies.
References:
1. Yao, Dianbo, Dai, Wanlin, Dong, Ming, Dai, Chaoliu, Wu, Shuodong. 2021. MUC2 and related bacterial factors: Therapeutic targets for ulcerative colitis. In EBioMedicine, 74, 103751. doi:10.1016/j.ebiom.2021.103751. https://pubmed.ncbi.nlm.nih.gov/34902790/
2. Liu, Yaxin, Yu, Zihan, Zhu, Lanping, Guli, Sitan, Chen, Xin. 2023. Orchestration of MUC2 - The key regulatory target of gut barrier and homeostasis: A review. In International journal of biological macromolecules, 236, 123862. doi:10.1016/j.ijbiomac.2023.123862. https://pubmed.ncbi.nlm.nih.gov/36870625/
3. Gundamaraju, Rohit, Chong, Wai Chin. 2021. Consequence of distinctive expression of MUC2 in colorectal cancers: How much is actually bad? In Biochimica et biophysica acta. Reviews on cancer, 1876, 188579. doi:10.1016/j.bbcan.2021.188579. https://pubmed.ncbi.nlm.nih.gov/34139275/
4. Allen, A, Hutton, D A, Pearson, J P. . The MUC2 gene product: a human intestinal mucin. In The international journal of biochemistry & cell biology, 30, 797-801. doi:. https://pubmed.ncbi.nlm.nih.gov/9722984/
5. Liu, Yang, Yu, Xinjie, Zhao, Jianxin, Zhai, Qixiao, Chen, Wei. 2020. The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. In International journal of biological macromolecules, 164, 884-891. doi:10.1016/j.ijbiomac.2020.07.191. https://pubmed.ncbi.nlm.nih.gov/32707285/
6. Levi, E, Klimstra, D S, Andea, A, Basturk, O, Adsay, N V. . MUC1 and MUC2 in pancreatic neoplasia. In Journal of clinical pathology, 57, 456-62. doi:. https://pubmed.ncbi.nlm.nih.gov/15113850/
7. Astashchanka, Anna, Shroka, Thomas M, Jacobsen, Britta M. 2018. Mucin 2 (MUC2) modulates the aggressiveness of breast cancer. In Breast cancer research and treatment, 173, 289-299. doi:10.1007/s10549-018-4989-2. https://pubmed.ncbi.nlm.nih.gov/30317423/
8. Zhou, Hanyu, Zhu, Yingwei, Wei, Feifei, Xu, Kequn, Cheng, Yuqing. 2019. Significance of MUC2 gene methylation detection in pancreatic cancer diagnosis. In Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 19, 1049-1053. doi:10.1016/j.pan.2019.09.012. https://pubmed.ncbi.nlm.nih.gov/31590960/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen