C57BL/6JCya-Myo1cem1/Cya
Common Name:
Myo1c-KO
Product ID:
S-KO-03311
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Myo1c-KO
Strain ID
KOCMP-17913-Myo1c-B6J-VA
Gene Name
Product ID
S-KO-03311
Gene Alias
MMIb; MYO1E; NMI; mm1beta; myr2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myo1cem1/Cya mice (Catalog S-KO-03311) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108431
NCBI RefSeq
NM_001080775
Target Region
Exon 2~14
Size of Effective Region
~5.2 kb
Detailed Document
Overview of Gene Research
Myo1c, also known as myosin1c, is an unconventional myosin motor protein. It is involved in various cellular processes such as cytoskeleton remodeling, intracellular transport, and vesicle tethering, which are crucial for normal cell function and tissue homeostasis. It is associated with pathways like TGF-β signaling, and its functions impact diverse biological processes in multiple tissues [1,2]. Genetic models, including gene knockout models, have been instrumental in understanding Myo1c's functions.
In podocyte-specific Myo1c knockout mice, resistance to fibrotic injury induced by Adriamycin or nephrotoxic serum was observed, suggesting Myo1c's role in TGF-β-signaling-mediated podocyte disease pathogenesis [2]. Systemic loss of Myo1c in mice led to rhodopsin mislocalization and impaired visual function [3]. In zebrafish, Myo1c knockdown caused abnormal podocyte morphology, absence of the slit diaphragm, and altered glomerular filter permeability, indicating its necessity for podocyte morphogenesis [4].
In conclusion, Myo1c is essential for multiple biological functions, including maintaining normal podocyte function, visual function, and glomerular development. Gene knockout models in mice and zebrafish have been pivotal in revealing its role in fibrotic diseases, visual impairment, and podocyte-related pathologies, providing insights into potential therapeutic targets for these conditions.
References:
1. Liu, Di, Li, Ruiru, Xu, Siqi, Xiao, Youjun, Xu, Hanshi. 2022. SMOC2 promotes aggressive behavior of fibroblast-like synoviocytes in rheumatoid arthritis through transcriptional and post-transcriptional regulating MYO1C. In Cell death & disease, 13, 1035. doi:10.1038/s41419-022-05479-0. https://pubmed.ncbi.nlm.nih.gov/36513634/
2. Arif, Ehtesham, Solanki, Ashish K, Srivastava, Pankaj, Kwon, Sang-Ho, Nihalani, Deepak. 2019. The motor protein Myo1c regulates transforming growth factor-β-signaling and fibrosis in podocytes. In Kidney international, 96, 139-158. doi:10.1016/j.kint.2019.02.014. https://pubmed.ncbi.nlm.nih.gov/31097328/
3. Solanki, Ashish K, Biswal, Manas R, Walterhouse, Stephen, Nihalani, Deepak, Lobo, Glenn Prazere. 2021. Loss of Motor Protein MYO1C Causes Rhodopsin Mislocalization and Results in Impaired Visual Function. In Cells, 10, . doi:10.3390/cells10061322. https://pubmed.ncbi.nlm.nih.gov/34073294/
4. Arif, Ehtesham, Kumari, Babita, Wagner, Mark C, Holzman, Lawrence B, Nihalani, Deepak. 2013. Myo1c is an unconventional myosin required for zebrafish glomerular development. In Kidney international, 84, 1154-65. doi:10.1038/ki.2013.201. https://pubmed.ncbi.nlm.nih.gov/23715127/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen